Tyrosine Phosphorylation Proteomics Analysis Service
In the realm of targeted protein therapy for cancer treatment, kinases, particularly tyrosine kinases (TKs), are abundant and play a crucial role. The development of Tyrosine Kinase Inhibitors (TKIs) necessitates a thorough analysis of their effects on the kinase activity spectrum.
In contrast to traditional in vitro kinase spectrum screening, phosphoproteomics provides a method that doesn’t require pre-selecting specific target proteins for detection. This technique enables a more comprehensive and accurate monitoring of kinase activity changes within living cells, identifying and quantifying specific protein phosphorylation sites.
Phosphoproteomics offers a direct approach to analyzing kinase activity within cells. When combined with traditional in vitro kinase spectrum screening, it enhances the accuracy and efficiency of assessing the targeting efficacy of kinase inhibitors under development. It provides comprehensive information on off-target effects, incorrect targeting, and multi-target effects. However, the content of phosphorylation, especially tyrosine phosphorylation (pY), is extremely low, necessitating deep enrichment techniques for relevant omics analysis.
Why Choose Kinase Insight
At Kinase Insight, we leverage our Tyrosine Phosphorylation Proteomics Technology platform to provide analytical support for the development of tyrosine kinase inhibitor drugs (TKIs). This platform accurately and directly confirms the targeting performance of kinase inhibitors in cells, predicting the effectiveness of innovative drugs under development. Additionally, we conduct hypothesis-free, direct intracellular analysis of the targeting capabilities of inhibitors under development, particularly regarding potential multi-targeting and off-target scenarios.
Workflow
1. Protein extraction from cancer cell lines or PDX models under different drug treatments.
2. Protein digestion.
3. Tyrosine phosphorylation peptide enrichment / tyrosine phosphorylation & serine phosphorylation peptide enrichment.
4. High-resolution liquid chromatography-mass spectrometry data acquisition.
5. Bioinformatics analysis.