ADP-ribosylation Analysis Service
ADP-ribosylation is an important post-translational modification (PTM) in which ADP-ribosyltransferases (ARTs) or poly(ADP-ribose) polymerases (PARPs) use NAD⁺ as the substrate to transfer ADP-ribose moieties to specific sites on proteins. According to the modification form, ADP-ribosylation can be divided into mono-ADP-ribosylation (MARylation) and poly-ADP-ribosylation (PARylation).
Suskiewicz, MJ. et al. Cell. 2023.
This modification plays a key role in cellular regulation, participating in DNA damage repair, transcriptional regulation, signal transduction, stress responses, and immune modulation. Abnormal ADP-ribosylation levels are associated with multiple diseases, especially cancer, inflammation, and neurodegenerative disorders. In recent years, drugs targeting ADP-ribosylation (such as PARP inhibitors) have become an important direction in precision medicine.
As a professional CRO specializing in proteomics, MtoZ Biolabs provides a high-quality ADP-ribosylation analysis service based on advanced mass spectrometry platforms and well-established detection schemes. Our services cover identification, quantification, and validation of the modification, helping researchers to elucidate the molecular mechanisms of ADP-ribosylation and its value in disease research and drug development.
Services at MtoZ Biolabs
MtoZ Biolabs' ADP-ribosylation analysis service is centered on mass spectrometry and combined with structural biology methods to provide a systematic research plan, mainly including the following aspects:
1. Target Protein ADP-ribosylation Analysis
MtoZ Biolabs provides Target Protein ADP-ribosylation Analysis, focusing on determining whether a specific protein undergoes ADP-ribosylation, including mono-ADP-ribosylation (MAR) and poly-ADP-ribosylation (PAR), and precisely mapping its modification sites. ADP-ribosylation is low in abundance, structurally complex, and prone to degradation, which makes it difficult to study. Through immunoprecipitation, anti-ADP-ribose antibody enrichment, Af1521 macrodomain capture, or PAR and MAR selective recognition modules, we selectively isolate the target protein from complex biological samples and use high-resolution LC-MS/MS to accurately identify the amino acid residues that carry the modification.
2. ADP-ribosylation Proteomics Analysis
MtoZ Biolabs provides systematic ADP-ribosylation Proteomics Analysis that covers proteome-wide MAR and PAR events. This service integrates anti-ADP-ribose antibody enrichment, Af1521 motif capture, PARP activity-related enrichment strategies, and multidimensional fractionation approaches such as HILIC and SCX, combined with high-resolution LC-MS/MS and multiple fragmentation modes, allowing the identification and quantification of large numbers of ADP-ribosylation sites within a single workflow.
Analysis Workflow
Hendriks, IA. et al. Mol Cell Proteomics. 2019.
Service Advantages
1. Optimized protocols
ADP-ribosylation is unstable and prone to hydrolysis, placing higher demands on experimental design. In sample pretreatment and detection, MtoZ Biolabs uses workflows optimized for this modification to minimize loss and ensure result reliability.
2. Multi-level information
Our ADP-ribosylation analysis service is not limited to preliminary identification of modified proteins; it also provides modification sites, modification forms (mono- or poly-ADP-ribosylation), and trends under different conditions. This multi-dimensional information helps researchers understand the role of ADP-ribosylation from the whole to the details.
3. Adaptable to different research scales
Whether for exploratory small-scale studies or large-sample systematic analyses, MtoZ Biolabs can flexibly design experimental workflows. We provide matched detection schemes for basic research, disease mechanism studies, and drug development to efficiently achieve research goals.
4. One-Time Charge
Our pricing is transparent, with no hidden fees or additional costs.
Applications
✔️ DNA damage repair research: Elucidate the role of PARP-mediated poly-ADP-ribosylation in repair processes.
✔️ Cancer research: Reveal abnormal patterns of ADP-ribosylation in tumor development and support target discovery.
✔️ Drug development: Provide experimental data for mechanism studies and efficacy evaluation of PARP inhibitors and other drugs.
✔️ Immunity and inflammation research: Study the role of ADP-ribosylation in inflammatory signaling regulation and immune responses.
✔️ Protein function regulation: Explore the effects of ADP-ribosylation on protein stability, interactions, and activity.
With advanced mass spectrometry platforms and a professional team, MtoZ Biolabs provides a high-quality and reliable ADP-ribosylation analysis service to researchers. If you are studying ADP-ribosylation or related molecular mechanisms, please contact us and let us provide comprehensive support for your research projects.