Venom Glycoproteomics Service

Venom glycoproteomics integrates mass spectrometry, glycomics, and bioinformatics methods to systematically identify and quantify glycosylation modifications in venom proteins, providing data support for revealing the molecular functions, evolutionary mechanisms, and potential pharmacological effects of venom. It is not only a key component of venomics but also an essential foundation for antivenom drug development, biopharmaceutical optimization, and structural biology research.

 

With extensive proteomics research experience and advanced high-resolution mass spectrometry platforms, MtoZ Biolabs has established a comprehensive Venom Glycoproteomics Service that enables in-depth characterization of glycan modifications in venom glycoproteins. Through integrated qualitative, quantitative, and structural analyses, we help research institutions and enterprises explore the biological significance and application potential of venom molecular modifications.

 

Services at MtoZ Biolabs

The venom glycoproteomics service at MtoZ Biolabs provides a full analytical workflow from glycan release and glycopeptide detection to glycoprotein-level characterization. Based on high-resolution liquid chromatography–mass spectrometry (LC-MS/MS) and multiple glycan enrichment and identification strategies, we offer comprehensive and systematic venom glycoprotein analysis services. The main services include:

1. Venom N-Glycan Analysis Service

MtoZ Biolabs provides Venom N-Glycan Analysis Service to deliver high-resolution qualitative and quantitative profiling of venom-derived N-glycans. By integrating optimized enzymatic release protocols, advanced HILIC-UHPLC MS and MALDI-TOF MS platforms, and expert bioinformatics pipelines, we enable accurate identification, and quantification of glycans in diverse venom samples.

 

2. Venom N-Glycan Quantitative Analysis Service

MtoZ Biolabs provides Venom N-Glycan Quantitative Analysis Service to accurately quantify N-glycan structures from venom proteins with high sensitivity and reproducibility. Using isotope and isobaric labeling strategies for relative quantification, combined with chemically pure glycan standards for absolute quantification, we ensure precise measurement of glycan abundance and heterogeneity. Our integrated workflow with advanced MS and HILIC-UHPLC platforms delivers reliable quantitative data, supporting venom research, toxin mechanism studies, antivenom development and related applications.

 

3. Venom N-Glycan Qualitative Analysis Service

MtoZ Biolabs provides Venom N-Glycan Quantitative Analysis Service to accurately quantify N-glycan structures from venom proteins with high sensitivity and reproducibility. Using isotope and isobaric labeling strategies for relative quantification, combined with chemically pure glycan standards for absolute quantification, we ensure precise measurement of glycan abundance and heterogeneity. Our integrated workflow with advanced MS and HILIC-UHPLC platforms delivers reliable quantitative data, supporting venom research, toxin mechanism studies, antivenom development and related applications.

 

4. Venom O-Glycan Analysis Service

Relying on its advanced mass spectrometry-based proteomics platform, MtoZ Biolabs provides the Venom O-Glycan Analysis Service which enables comprehensive characterization and quantitative analysis of O-glycans from various venom sources, including snake venom, spider venom, scorpion venom, and marine toxins, offering systematic and reliable data support for research institutions and enterprises to better understand the biological significance and potential applications of venom O-glycans.

 

5. Venom Glycopeptides Analysis Service

The Venom Glycopeptides Analysis Service at MtoZ Biolabs aims to comprehensively analyze the composition, modification types, and functional correlations of glycopeptides in venom. By integrating high-resolution liquid chromatography–mass spectrometry (LC-MS/MS), glycopeptide-specific enrichment strategies, and multidimensional data analysis workflows, we provide end-to-end analytical support from sample preparation to data interpretation.

 

MtoZ Biolabs can design customized experimental and data analysis plans tailored to different venom types, including snake, spider, scorpion, insect, and marine venoms.

 

Analysis Workflow

1. Sample preparation and pretreatment

Venom proteins are extracted, quantified, and enzymatically digested, followed by desalting or concentration to remove impurities.

 

2. Glycan release and enrichment

Depending on the sample type, enzymatic or chemical methods are used for glycan release, while HILIC, lectin, or PGC materials are employed to enrich glycans or glycopeptides.

 

3. High-resolution mass spectrometry detection

Analysis is performed on Orbitrap Fusion Lumos and Q Exactive HF-X systems with multiple fragmentation strategies such as HCD, EThcD, and CID.

 

4. Data identification and annotation

Specialized software such as Byonic, pGlyco, and GlycoWorkbench is used for glycan structure identification, modification site annotation, and database comparison.

 

5. Results and reporting

Structural annotation tables, abundance statistics, glycan distribution charts, and structural maps are provided along with data interpretation and further research recommendations.

 

Figure 1. Workflow for Venom Glycoproteomics

 

Service Advantages

1. High resolution

MtoZ Biolabs is equipped with multiple high-performance Orbitrap and Q-TOF mass spectrometers combined with Nano-LC systems, enabling highly sensitive detection of low-abundance glycoproteins in complex venom samples while ensuring data quality and accuracy.

 

2. Comprehensive coverage

Our venom glycoproteomics service covers multiple layers of glycosylation analysis, including N-glycans, O-glycans, glycopeptides, and glycoproteins, allowing flexible combinations of analytical strategies based on sample type and research objectives to achieve complete glycoprotein profiling.

 

3. Multiple quantification modes

We support TMT, DIA, Label-Free, and PRM quantification methods suitable for exploratory studies, comparative analyses, and targeted validation, providing multidimensional quantitative data for venom glycosylation research.

 

4. Professional team

Our team has long-standing experience in glycomics, proteomics, and venom biology, with expertise in structural analysis and functional interpretation of glycosylation modifications, ensuring scientifically sound and interpretable results.

 

Applications

✔️ Venom functional and structural mechanism studies: analyzing the effects of glycosylation on toxin activity, stability, and receptor recognition

✔️ Antivenom and antibody development: identifying key glycosylation sites to support antibody neutralization and drug target studies

✔️ Venom evolution and comparative studies: comparing glycoprotein differences among species or environmental conditions to explore evolutionary adaptation mechanisms

✔️ Biomarker discovery: identifying specific glycan structures or modification patterns for species identification or venom quality evaluation

✔️ Structural biology research: providing glycan structure and site information for toxin 3D modeling and functional studies

 

Sample Submission Suggestions

1. Sample types: natural venom samples or recombinant venom proteins, including snake, spider, scorpion, insect, and marine venoms

2. Sample amount: total protein ≥100 µg, concentration ≥1 µg/µL

3. Storage: store at −80°C and avoid repeated freeze-thaw cycles

4. Transportation: ship on dry ice and maintain low temperature during transport

5. Buffer requirements: avoid high salt, glycerol, detergents, or other interfering components

6. Additional information: provide sample source, extraction method, concentration, and research objective for optimized analysis design

 

MtoZ Biolabs provides detailed sample submission guidelines and packaging instructions to ensure sample stability and analytical suitability during transport.

 

Deliverables

1. Standardized analysis report

2. Raw data files

3. Statistical and annotation tables

4. Visual figures

5. Summary and interpretation

 

Contact our technical experts to obtain a customized venom glycoproteomics analysis solution and make MtoZ Biolabs your trusted partner in scientific research.