Integrative PTMs and Metabolomics Analysis Service
Protein post-translational modifications (PTMs) are key mechanisms for regulating protein function and signaling pathways, while metabolites are the most direct energy and material basis of cellular activities. In recent years, studies have shown that metabolites exist not only as energy and intermediates but also directly act as donors or regulators of PTMs, deeply participating in the regulation of protein functions. For example, acetyl-CoA participates in acetylation, succinyl-CoA drives succinylation, and glutathione is closely related to S-nitrosylation and sulfhydration. This "metabolite–PTM–enzyme" coupled network has become an important mechanism for cellular homeostasis and disease occurrence.
Integrative PTMs and metabolomics analysis service is based on this scientific background, combining PTMs analysis with metabolomics detection to comprehensively reveal the interactions between metabolic states and PTMs. By simultaneously obtaining protein modification profiles and metabolite profiles, researchers can not only understand how metabolites drive specific modification events but also explore how PTMs regulate metabolic pathways in return.
As a professional proteomics CRO, MtoZ Biolabs relies on advanced high-resolution mass spectrometry platforms and systematic metabolite detection capabilities to provide a high-quality integrative PTMs and metabolomics analysis service for global researchers and pharmaceutical companies. Our services help clients obtain more comprehensive evidence in disease mechanism studies, drug development, and biomarker discovery, promoting the transformation of research findings into practical value.
Services at MtoZ Biolabs
1. PTMs Detection
- Covering multiple common modifications: phosphorylation, acetylation, methylation, ubiquitination, glycosylation, lipidation, S-nitrosylation, etc.
- Achieving site-level precise quantification through optimized peptide enrichment methods and high-resolution LC-MS/MS detection.
2. Metabolite Analysis
- Covering core pathways such as energy metabolism, amino acid metabolism, lipid metabolism, and redox metabolism.
- Quantitative comparison of disease-related metabolites and oncometabolites.
3. Integrative PTMs and Metabolomics Analysis
- Cross-comparing modification levels with metabolite changes to identify metabolite-driven PTM events.
- Constructing PTM–metabolite interaction networks to reveal regulatory patterns under metabolic reprogramming.
- Using advanced bioinformatics tools for pathway enrichment, kinase–substrate prediction, and functional network analysis.
4. Customized Analysis Solutions
- Selecting different types of modifications and metabolic pathways according to research objectives.
- Supporting integration with other omics (such as transcriptomics and global proteomics) to expand interpretation depth.
Analysis Workflow
1. Sample preparation: protein extraction, modified peptide enrichment, and metabolite extraction.
2. Liquid chromatography separation: efficient separation of peptides and metabolites.
3. High-resolution mass spectrometry detection: parallel LC-MS/MS detection of PTMs and metabolites.
4. Data processing and comparison: identification and quantification of sites and metabolites.
5. Multi-omics integration: establishing metabolite–PTMs networks and performing functional annotation with pathway databases.
6. Report delivery: generating systematic analysis results and visual charts.
Nalbantoglu, S. et al. J Pharm Biomed Anal. 2021.
Figure 2. Integrative PTMs and Metabolomics Analysis
Service Advantages
1. Comprehensive Molecular Insight: Integrating modification proteomics and metabolomics to reveal causal relationships of molecular regulation.
2. High-Resolution Detection: Our integrative PTMs and metabolomics analysis service ensures high-resolution detection based on advanced LC-MS/MS platforms for accurate identification and quantification of PTMs and metabolites.
3. Customized Panels: Options to analyze combinations of phosphorylation, acetylation, ubiquitination, and core metabolic pathways according to needs.
4. Advanced Data Integration: Multi-level data integration process to help build actionable biological hypotheses.
5. Flexible Applicability: Supporting both small-scale exploratory studies and large-scale cohort studies, covering different research requirements.
Applications
- Disease Mechanism Research: Revealing the coupling mechanisms of metabolites and PTMs in cancer, diabetes, cardiovascular diseases, etc.
- Drug Mechanism of Action: Verifying how drugs act on signaling pathways through PTMs and metabolism.
- Biomarker Discovery: Jointly using PTMs and metabolites as composite biomarkers to improve the specificity of diagnosis and prognosis prediction.
- Systems Biology Research: Constructing a complete cellular regulatory network and exploring the intersections of metabolic reprogramming and signaling regulation.
FAQs
Q1: Is a small sample size feasible?
A1: Yes. We have optimized enrichment and detection workflows to obtain reliable results even with limited samples.
Q2: Can cross-omics integration be performed?
A2: Yes. Our platform supports multi-omics integration with transcriptomics, proteomics, and others.
Sample Submission Suggestions
| Sample Type | Minimum Requirement | Notes |
| Protein sample | ≥100 μg | Avoid contamination of salts and detergents |
| Cell sample | ≥1×10⁷ cells | Keep cells in good condition, avoid excessive dead cells |
| Tissue sample | ≥200 mg | Store at -80 ℃, avoid repeated freeze–thaw cycles |
| Blood/Plasma | ≥1 mL | Collect with anticoagulant, transport on dry ice |
| Serum | ≥0.5 mL | Avoid multiple freeze–thaw cycles |
| Urine | ≥2 mL | Freeze immediately if possible |
Deliverables
1. Experimental report (including quality control and experimental conditions)
2. PTMs identification and quantification list
3. Metabolite qualitative and quantitative results
4. Multi-omics integration analysis report (network models, pathway enrichment, functional annotation)
5. Data visualization (heatmaps, pathway maps, network diagrams, etc.)
6. Raw mass spectrometry data files
Integrative PTMs and metabolomics analysis service combines modification proteomics and metabolomics to provide researchers with a new perspective for deeply understanding cellular regulatory networks. With strong platforms and a professional team, MtoZ Biolabs provides systematic support for your research and drug development. Contact us to obtain customized integrative solutions and accelerate your research toward new breakthroughs.