Methylation Site Identification Service
Accurate identification of methylation sites is essential for understanding how proteins are regulated, how chromatin states are maintained, and how cellular pathways respond to developmental or environmental cues. Methylation Site Identification Service at MtoZ Biolabs helps researchers pinpoint exact lysine and arginine residues that undergo mono-, di-, or trimethylation, uncover the biological consequences of these modifications, and generate high-value data for epigenetics, cancer research, and biomarker discovery.
Overview
Methylation is a key post-translational modification that occurs on lysine (Lys) and arginine (Arg) residues. Protein methyltransferases catalyze the addition of methyl groups to specific amino acids, generating mono-, di-, or trimethylated forms, while demethylases remove them, allowing dynamic regulation.
Histone methylation is central to transcriptional activation and repression, chromatin remodeling, and epigenetic memory. Non-histone methylation is increasingly recognized for its role in modulating enzyme activity, signal transduction, DNA repair, and immune function. Because these modifications often occur at low abundance and in complex regulatory contexts, mapping methylation sites with precision provides critical insights into cellular control mechanisms and disease pathology.
Services at MtoZ Biolabs
1. Targeted Methylation Site Identification
MtoZ Biolabs provides targeted identification of methylation sites on client-defined proteins. This service delivers precise residue-level characterization and quantitative assessment of selected targets and is suitable for focused studies involving specific regulatory proteins or defined experimental conditions.
2. Methylation Site Proteomics
MtoZ Biolabs offers proteome-wide profiling of methylation sites in complex biological samples. This service supports discovery-driven research and comparative analysis by mapping global methylation patterns across tissues, cell types, or treatment groups.
MtoZ Biolabs supports these services with high resolution Orbitrap platforms and established enrichment strategies such as immunoaffinity purification and hydrophilic interaction chromatography to ensure sensitive detection and accurate localization of methylated peptides. Computational assessments are available to assist with candidate site prediction and large-scale screening, providing an efficient complement to experimental analysis.
Guo, A. et al. Mol. Cell. Proteomics. 2014.
Figure 1. Methylation Site Identification Workflow by Mass Spectrometry
Why Choose MtoZ Biolabs?
✔ Advanced MS Platforms: Orbitrap Fusion, Lumos, and Q Exactive HF with Nano-LC deliver superior sensitivity and resolution.
✔ Diverse Enrichment Options: Immunoaffinity purification and HILIC provide broad coverage across Lys and Arg methylation.
✔ High-Confidence Site Localization: State-of-the-art algorithms minimize ambiguity in site assignment.
✔ Integrated Workflow: From enrichment to data analysis, ensuring reliability and reproducibility.
✔ Flexible Project Design: Customized strategies tailored to research focus and sample type.
✔ Transparent Pricing: One-time charge with no hidden costs.
Sample Submission Suggestions
Note: Provide details on sample collection and handling. If you need further details, our technical support team is happy to assist and provide comprehensive guidance on sample submission.
Applications
Our Methylation Site Identification Service supports a broad range of research areas and translational applications:
● Epigenetics
Identification of histone methylation sites to study chromatin remodeling, transcriptional regulation, and heritable epigenetic marks.
● Oncology and Clinical Research
Profiling aberrant methylation events that drive tumor initiation and progression, while also identifying site-specific methylation signatures with diagnostic and prognostic potential.
● Disease and Functional Studies
Mapping site-specific methylation in neuronal proteins, metabolic enzymes, and immune signaling proteins to uncover mechanisms underlying neurodegeneration, metabolic disorders, and host–pathogen interactions.
FAQ
Q1: Can methylation site identification be integrated with multi-PTM studies, such as phosphorylation or acetylation analysis?
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
Methylation Site Identification Service at MtoZ Biolabs enables researchers to explore the biological significance of protein methylation with confidence. Our expertise and integrated resources provide the foundation for advancing studies in epigenetics, disease research, and translational applications. In addition to methylation, MtoZ Biolabs delivers a comprehensive suite of PTM-omics services to support your discovery pipeline.
Contact us today to learn how our Methylation Site Identification Service can accelerate your research.