Glycopeptide Analysis Service

MtoZ Biolabs provides Glycopeptide Analysis Service that integrates optimized sample preparation, targeted enrichment strategies, and high-resolution LC-MS/MS platforms to deliver comprehensive characterization of protein glycosylation. Our solutions include glycopeptide identification, glycosylation site mapping, structural elucidation of glycan compositions and linkages, and quantitative profiling of glycoforms. By combining advanced instrumentation with expert bioinformatics pipelines, we ensure accurate, reproducible, and regulatory-compliant data that support both academic research and biopharmaceutical development.

 

Overview

Glycopeptides, generated by proteolytic digestion of glycoproteins, are unique analytical targets because they preserve both the peptide backbone and the covalently attached glycans. This dual information provides site-specific insight into protein glycosylation, linking glycan structures to their corresponding protein regions.

 

The analysis of glycopeptides is essential in biomedical and pharmaceutical research. In biopharmaceutical development, glycosylation is considered a critical quality attribute under regulatory guidelines such as ICH Q6B, as it directly affects drug safety, efficacy, and pharmacokinetics. Abnormal glycosylation patterns have also been associated with cancers, autoimmune disorders, neurodegenerative diseases, and infectious diseases. Therefore, detailed characterization of glycopeptides is vital not only for fundamental biology but also for clinical and therapeutic applications.

 

de Haan N. et al. Nat Rev Chem. 2020.

Figure 1. Glycopeptide Analysis Workflow

 

Analysis Workflow

The general analytical workflow for the Glycopeptide Analysis Service is as follows:

 

1. Sample Preparation and Quality Control

Proteins from biological or pharmaceutical samples are evaluated for concentration and purity. Buffer exchange or cleanup is performed when necessary to ensure compatibility with downstream analysis.

 

2. Enzymatic Digestion

Proteins are digested with proteases such as trypsin, or combinations of enzymes, to generate peptides and glycopeptides while preserving glycosylation integrity.

 

3. Glycopeptide Enrichment

Selective enrichment methods, such as lectin affinity, HILIC, are applied to increase the relative abundance of glycopeptides and reduce interference from non-glycosylated peptides.

 

4. LC-MS/MS Analysis

Enriched glycopeptides are analyzed using high-resolution LC-MS/MS platforms to reveal both glycan structures and peptide backbones.

 

5. Data Processing and Bioinformatics

Spectral data are interpreted using curated glycan databases and specialized software to assign glycan compositions, localize glycosylation sites, and quantify glycoform distributions.

 

6. Reporting

Clients receive a comprehensive report including experimental details, annotated glycopeptide lists, glycosylation site assignments, structural information, quantitative data, and interpretation.

 

Service Advantages

1. Advanced Analysis Platform

MtoZ Biolabs established an advanced Glycopeptide Analysis Service platform, guaranteeing reliable, fast, and highly accurate analysis service.

 

2. Comprehensive Analytical Coverage

From glycopeptide identification and glycosylation site mapping to structural elucidation and quantitative profiling, we provide end-to-end solutions.

 

3. Expert Bioinformatics Integration

Curated glycan databases and specialized algorithms deliver confident assignments, reproducible quantification, and biologically meaningful interpretation.

 

4. Regulatory and Application-Oriented Outputs

Standardized workflows and strict quality control provide reproducible results aligned with academic publishing requirements and regulatory guidelines for biopharmaceutical development.

 

Sample Submission Suggestions

 

It is recommended to contact our technical support team before submitting samples to determine sample suitability and obtain tailored submission guidelines.

Applications

Practical applications of Glycopeptide Analysis Service are as follows:

 

1. Monoclonal Antibody Development

Characterize Fc glycosylation patterns that influence antibody-dependent cellular cytotoxicity (ADCC), pharmacokinetics, and immunogenicity.

 

2. Biosimilarity Assessment

Compare glycopeptide profiles between reference and biosimilar products to demonstrate structural equivalence and meet regulatory requirements.

 

3. Vaccine Glycoprotein Characterization

Analyze glycosylation of viral antigens to inform immunogenicity, vaccine design, and stability studies.

 

4. Site-Directed Mutagenesis Studies

Confirm changes in glycosylation occupancy and structure following mutagenesis of glycosylation sites.

 

5. Process Monitoring and Batch Consistency

Evaluate glycosylation heterogeneity across manufacturing batches as a critical quality attribute in biopharmaceutical production.

 

FAQ

Q1: Can Both N-linked and O-linked Glycopeptides Be Analyzed?

A1: Yes. Our enrichment strategies, including lectin affinity, HILIC, and hydrazide chemistry, in combination with LC-MS/MS, enable sensitive and specific analysis of both N-linked and O-linked glycopeptides.

 

Q2: Can the Service Provide Quantitative Glycoform Distribution Data?

A2: Yes. Relative quantification of glycoforms is performed using specialized bioinformatics pipelines, enabling comparison of glycosylation patterns across biological conditions, production batches, or therapeutic candidates.