SUMO Proteomics Analysis Service
SUMO proteomics is an important field that studies the modification characteristics, dynamic changes, and biological functions of Small Ubiquitin-like Modifier (SUMO) proteins at the proteome level. SUMOylation is a reversible post-translational modification (PTM) in which a SUMO protein is covalently attached to a lysine residue of a substrate protein, regulating protein stability, localization, interaction, and activity. Unlike ubiquitination, SUMOylation does not directly label proteins for degradation but is widely involved in cell cycle regulation, DNA repair, transcriptional regulation, stress response, and signal transduction.
Le, NT. et al. Int J Inflam. 2012.
With the rapid advancement of mass spectrometry technology, SUMO proteomics analysis has become a key approach for deciphering protein post-translational modification networks. Systematic identification and quantification of SUMOylated proteins and their modification sites help to reveal cellular response mechanisms, disease-related signaling pathways, and potential drug targets.
With advanced LC-MS/MS platforms, including Orbitrap Fusion Lumos, Q Exactive HF, and timsTOF Pro, as well as a comprehensive bioinformatics analysis system, MtoZ Biolabs has established a highly sensitive SUMO Proteomics Analysis Service that covers SUMOylated peptide enrichment, modification site identification, quantitative analysis, and systematic data interpretation, providing researchers with an end-to-end solution from experimentation to data analysis to support in-depth exploration of SUMOylation mechanisms.
Services at MtoZ Biolabs
1. SUMOylated Peptide Enrichment Service
SUMOylated proteins are typically of low abundance in cells, and the modification is both reversible and dynamic. Thus, peptide enrichment prior to mass spectrometry analysis is particularly important to selectively capture SUMOylated peptides, enhance detection sensitivity, and improve site identification accuracy. Leveraging advanced proteomics platforms and extensive experience in PTM research, MtoZ Biolabs offers professional SUMOylated peptide enrichment service to support researchers in comprehensive SUMOylation analysis.
2. SUMOylation Site Identification Service
Relying on advanced mass spectrometry platforms and extensive PTM research experience, MtoZ Biolabs offers a comprehensive SUMOylation site identification service that covers sample preparation, SUMOylated peptide enrichment, MS detection, and bioinformatic analysis to help researchers systematically explore the regulatory roles of SUMOylation in cellular physiology and pathology.
3. Quantitative SUMO Proteomics Service
Relying on advanced LC–MS/MS analytical platforms and systematic quantitative proteomics programs, MtoZ Biolabs provides high-quality Quantitative SUMO Proteomics Service for global research institutions and pharmaceutical companies. We use multiple quantitative modes, including SILAC, iTRAQ/TMT, Label-Free, and DIA/SWATH, to provide customers with flexible, reliable SUMO proteomics research solutions, helping promote in-depth progress in scientific research and drug development.
4. SUMO Proteomics Data Analysis Service
MtoZ Biolabs has established a systematic SUMO proteomics data analysis service that combines standardized data processing workflows with multi-dimensional bioinformatics analysis to provide professional SUMO modification data interpretation solutions for research institutions and pharmaceutical companies. Our service helps clients extract meaningful information from complex mass spectrometry data, identify SUMO modification sites, quantify dynamic changes, and reveal potential regulatory networks, thereby accelerating mechanistic research and drug development.
Analysis Workflow
1. Sample Preparation: Extract total proteins from cells or tissues after lysis, followed by quantification and quality assessment.
2. Digestion and Enrichment: After enzymatic digestion with trypsin, SUMOylated peptides are enriched using immunoaffinity or chemical strategies.
3. Mass Spectrometry Detection: Perform LC-MS/MS analysis to acquire high-resolution peptide spectra.
4. Data Processing: Identify SUMOylation sites through multi-engine database searches, characteristic residue filtering, and manual validation.
5. Bioinformatics Analysis: Integrate differential expression, pathway analysis, and functional annotation to generate visualized analytical reports.
Figure 2. Workflow of SUMO Proteomics Analysis
Service Advantages
1. Specific Enrichment
SUMO modification signals are often extremely weak. MtoZ Biolabs combines Anti-SUMO Antibody Immunoprecipitation (IP), Tag-Based Affinity Enrichment, and Peptide-Level Enrichment After Enzymatic Digestion to significantly enhance the detection rate and specificity of SUMOylated peptides. By optimizing antibody recognition, tag affinity, and QTGG motif-based enrichment conditions, we achieve high-confidence detection of low-abundance SUMOylated proteins.
2. High-Resolution Mass Spectrometry Platforms
Our platform includes internationally recognized instruments such as Orbitrap Fusion Lumos, Q Exactive HF, and timsTOF Pro, enabling flexible matching of sample types and experimental goals. The complementary multi-platform strategy ensures wide dynamic range and high resolution, meeting the demands of complex and low-abundance modification analysis.
3. Experienced Team
MtoZ Biolabs has an expert team with extensive experience in post-translational modification proteomics and follows strict quality control standards. Each project includes internal QC samples and standard curves to ensure data stability and traceability.
4. One-Time Charge
Our pricing is transparent, with no hidden fees or additional costs.
Applications
1. Cell Stress and DNA Repair Research: Reveal the regulatory mechanisms of SUMOylation in oxidative stress, heat shock, and DNA damage response.
2. Cancer and Neurological Disease Research: Explore the roles of SUMOylated proteins in cancer signaling pathways, neurodegenerative diseases, and inflammation.
3. Transcriptional Regulation and Protein Interaction Analysis: Investigate how SUMOylation affects transcription factor activity and protein complex dynamics.
4. Drug Target and Mechanism Studies: Identify SUMO-related regulatory nodes and potential therapeutic targets.
Sample Submission Suggestions
1. Sample Type: Cells, tissues, recombinant proteins, or purified samples are acceptable.
2. Sample Condition: Provide lyophilized powder or frozen lysate; avoid repeated freeze-thaw cycles.
3. Sample Amount: For cell samples, at least 1×10⁷ cells are recommended; total protein amount ≥200 μg.
4. Buffer System: Avoid buffers containing high salt concentrations or strong ionic detergents.
5. Additional Information: Please include sample treatment details, experimental grouping, and background information.
MtoZ Biolabs provides detailed sample submission guidelines based on project type to help clients optimize sample preparation.
Deliverables
1. Complete experimental scheme and QC report
2. List of SUMOylated proteins and modification sites
3. Differential analysis and statistical results
4. Functional annotation, pathway enrichment, and network visualization report
5. Raw MS data files and processed standardized data