Ubiquitomics Data Analysis Service

Ubiquitomics Data Analysis Service focuses on the comprehensive analysis of ubiquitination, a critical post-translational modification that regulates protein stability, degradation, localization, and signaling. By mapping ubiquitination sites and quantifying dynamic changes, this service provides essential insights into cellular proteostasis, disease mechanisms, and therapeutic target discovery.

 

At MtoZ Biolabs, we combine high-resolution mass spectrometry platforms with advanced bioinformatics pipelines to deliver accurate, reproducible, and biologically meaningful ubiquitomics datasets. Our Ubiquitomics Data Analysis Service is designed to support researchers from raw data interpretation to pathway-level insights, ensuring robust and publication-ready results.

 

Laigle, V. et al. Mol. Cell. Proteomics. 2021.

Figure 1. Multi-Layer Ubiquitomics Data Analysis Workflow

 

Services at MtoZ Biolabs

MtoZ Biolabs provides a comprehensive suite of ubiquitomics data analysis services, allowing clients to select the scope and depth of analysis based on project needs:

Raw Data Processing: Conversion of raw MS files, peak extraction, noise reduction, and calibration.

Protein Identification: Database search with diGly signature detection for ubiquitinated lysines.

Site Localization: High-confidence assignment of ubiquitination sites with statistical validation.

Quantitative Analysis: Options for label-free, SILAC, or TMT/iTRAQ-based quantification.

Polyubiquitin Chain Analysis: Characterization of linkage types (K48, K63, K11, linear, and others).

Bioinformatics Interpretation: GO/KEGG annotation, PPI network mapping, and E3 ligase prediction.

Multi-Omics Integration: Combining ubiquitomics with phosphorylation, acetylation, or other PTM datasets.

If you are not satisfied with the quality or completeness of your existing datasets, MtoZ Biolabs also provides end-to-end ubiquitomics solutions, covering sample preparation, mass spectrometry analysis, and comprehensive data interpretation, ensuring accurate site identification and high-quality outputs.

 

Why Choose MtoZ Biolabs?

✔ Advanced Platforms: Proven expertise in handling high-resolution Orbitrap and Q-TOF MS data.

✔ Comprehensive Coverage: Site-specific ubiquitination, linkage types, and cross-PTM integration.

✔ Biological Insight: From raw data processing to pathway modeling and mechanistic interpretation.

✔ Tailored Solutions: Customized pipelines designed to match diverse project goals.

✔ One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

 

Sample/Data Submission Suggestions

MtoZ Biolabs accepts LC–MS/MS raw files (.raw, .mzML) or processed identification results. Clients are encouraged to provide experimental design details and metadata to ensure accurate interpretation. For complete submission guidelines, please contact our scientific team.

 

FAQ

Q1: Can ubiquitomics data analysis be integrated with other omics datasets?

Yes. We provide advanced bioinformatics pipelines that allow ubiquitomics datasets to be combined with phosphoproteomics, acetylomics, or transcriptomics data. This enables systems-level analysis, revealing PTM crosstalk and regulatory networks across multiple layers of cellular biology.

 

What Could be Included in the Report?

·  Comprehensive technical report with methodology, results, and interpretation

·  Identified ubiquitination sites with localization confidence scores

·  Quantitative datasets with differential analysis results

·  Graphical outputs: volcano plots, heatmaps, PPI networks, pathway enrichment diagrams

·  Raw and processed data files in standard formats (RAW, mzML, FASTA, Excel)

 

MtoZ Biolabs’ Ubiquitomics Data Analysis Service transforms complex MS datasets into actionable biological insights. By integrating robust quantification, polyubiquitin chain profiling, and advanced bioinformatics, we empower researchers to uncover novel mechanisms, validate therapeutic targets, and accelerate translational research. Contact our team today to learn how our ubiquitomics services can support your projects and complement our broader portfolio of post-translational proteomics solutions.