Post-Translational Modifications(PTM) Analysis Service

Post-translational modifications (PTMs) refer to chemical or enzymatic modifications that occur on specific amino acid residues or protein domains after protein translation is completed. These modifications are diverse, including phosphorylation, acetylation, methylation, ubiquitination, glycosylation, and redox modifications. PTMs not only alter the physicochemical properties of proteins but also regulate their spatial conformation, subcellular localization, molecular interactions, and functional states. They are essential processes in cellular signaling, metabolic regulation, and stress responses.

 

Zhong, Q. et al. MedComm (2020). 2023.

Figure 1. Common Types of Protein Posttranslational Modifications

  

Abnormal PTM patterns are closely associated with various diseases, including cancer, metabolic disorders, neurodegenerative diseases, and immune-related diseases. Therefore, systematic detection and analysis of PTMs are of great importance for elucidating molecular mechanisms, identifying disease biomarkers, evaluating drug action mechanisms, and promoting the development of novel therapeutic strategies.

 

With the advancement of high-resolution mass spectrometry and bioinformatics, mass spectrometry-based post-translational modifications analysis has become the core method for PTM research. It enables sensitive detection of modification types and sites, combined with quantitative strategies to reveal dynamic changes under different conditions, thus providing strong data support for life science and translational medicine research.

 

As a professional CRO company specialized in proteomics and mass spectrometry, MtoZ Biolabs provides comprehensive post-translational modifications (PTM) analysis service with advanced mass spectrometry platforms, standardized workflows, and an experienced research team. Our services cover multiple modification types and functional analysis, aiming to provide systematic and reliable research solutions for global academic and industrial clients.

 

Services at MtoZ Biolabs

MtoZ Biolabs provides systematic post-translational modifications (PTM) analysis service covering a wide range of common and specific PTMs:

 

Protein Phosphorylation Analysis Service	Protein Glycosylation Analysis Service	Protein Alkylation Analysis Service	Protein PTM Site Identification Service
Protein Ubiquitination Analysis Service	Protein SUMOylation Analysis Service	Protein Sulfation Analysis Service	N-Terminal PTM Analysis Service
Protein Methylation Analysis Service	Protein Deamidation Analysis Service	Protein PEGylation Analysis	Protein Backbone Post-translational Modifications Analysis
Protein Acetylation Analysis Service	Protein Oxidation Analysis Service	Protein Disulfide Bond Analysis Service	Protein Truncation Analysis Service

Analysis Workflow

1. Sample preparation: Clients provide suitable cells, tissues, or body fluid samples for protein extraction and pretreatment.

2. Modification enrichment: Various affinity or chemical methods are used to enrich target modified peptides.

3. Mass spectrometry detection: High-resolution LC-MS/MS is applied to acquire high-quality data.

4. Data processing: Raw data undergo quality control, peptide identification, and modification site localization.

5. Bioinformatics analysis: Quantitative analysis, functional enrichment, and pathway analysis are performed, generating visualized results.

 

Figure 2. Workflow for Post-Translational Modifications(PTM) Analysis 

 

Service Advantages

1. Advanced Platform

MtoZ Biolabs is equipped with high-resolution mass spectrometry systems (Orbitrap Fusion Lumos, Q Exactive HF, etc.), combined with Nano-LC systems, enabling sensitive detection of low-abundance modifications in complex samples and ensuring high-quality, deep-coverage data.

 

2. Experienced Team

Our team has extensive experience in proteomics and PTM research, capable of designing appropriate experimental strategies according to client needs, ensuring the reliability and interpretability of results.

 

3. One-Time Charge

Our pricing is transparent, no hidden fees or additional costs.

 

Applications

✔️ Basic research: Elucidating the role of PTMs in signal transduction, metabolic regulation, and gene expression.

✔️Disease research: Investigating the relationship between abnormal PTMs and cancer, neurological diseases, and immune disorders.

✔️ Drug development: Assessing the effects of candidate drugs on modification levels, supporting target discovery and validation.

✔️ Biopharmaceuticals: Structural characterization and functional validation of antibodies and protein drugs.

 

FAQs

Q1: Can the data be used for publication?

A1: Yes, we provide standardized analysis reports and raw data, which meet the requirements of scientific publication and industrial research.

 

Sample Submission Suggestions

• Protein samples: At least 100 μg

• Cell samples: Recommended 2 × 10⁷ cells

• Animal tissue: Recommended 1 g

• Plant tissue: Recommended 200 mg

• Blood samples (with EDTA anticoagulant): Recommended 1 mL

• Serum samples: Recommended 0.2–0.5 mL

• Urine samples: Recommended 2 mL

• Microbial samples: Recommended 200 mg (dry weight)

 

Deliverables

1. Detailed report of sample processing and experimental workflow

2. Raw mass spectrometry data files (e.g., .raw/.mzML)

3. Identification and quantification results of modification sites

4. Visualized charts of functional enrichment and pathway analysis

5. Standardized final analysis report (suitable for research and publication)

 

With advanced mass spectrometry platforms and a professional team, MtoZ Biolabs provides systematic post-translational modifications (PTM) analysis service. From modification detection to functional interpretation, we are committed to delivering reliable support for academic and industrial clients. Contact us to advance proteomics and life science research together.