Histone Post-Translational Modification Identification Service
Histones are essential structural proteins of eukaryotic chromatin. By binding to DNA, they form nucleosomes, which regulate the three-dimensional conformation of chromatin and the accessibility of genes. In recent years, numerous studies have demonstrated that histone post-translational modifications (PTMs) play central roles in gene transcription regulation, chromatin remodeling, DNA repair, and cell fate determination. Different types of modifications, such as acetylation, methylation, phosphorylation, and ubiquitination, can serve as signaling marks that influence chromatin accessibility, thereby regulating gene expression profiles.
Abnormal patterns of histone modifications are closely related to a variety of diseases, including tumorigenesis, neurodegenerative diseases, and metabolic disorders. Therefore, systematic and accurate identification of histone post-translational modifications is of great significance for elucidating epigenetic regulatory mechanisms, discovering potential disease biomarkers, and evaluating drug effects.
As a CRO company specializing in proteomics, MtoZ Biolabs provides histone post-translational modification identification service based on advanced mass spectrometry platforms and comprehensive sample preparation strategies. Our service helps researchers deeply analyze histone modification profiles, obtain high-quality and reproducible data, and provides solid technical support for epigenetic research, disease mechanism studies, and new drug development.
Services at MtoZ Biolabs
At MtoZ Biolabs, our histone post-translational modification identification service is based on mass spectrometry technology, combined with diverse sample preparation strategies and modified peptide enrichment methods, ensuring that researchers can comprehensively analyze histone modification types, modification sites, and dynamic changes.
Analysis Workflow
1. Histone extraction and purification
According to the sample type (cells, tissues, yeast, plants, etc.), acid extraction, high-salt extraction, or rapid chemical lysis methods are selected to maximally retain modification information. At the same time, chromatographic purification or protein precipitation is combined to remove impurities and improve histone purity and the reliability of downstream analyses.
2. Modified peptide preparation and enrichment
Proteolytic digestion strategies such as trypsin and Glu-C are applied to generate peptides, followed by selective enrichment of phosphorylated, acetylated, methylated, and other modified peptides using IMAC, TiO₂ enrichment, or immuno-affinity enrichment methods, improving the detection rate of low-abundance modifications.
3. High-resolution mass spectrometry detection
With high-resolution mass spectrometry platforms such as Orbitrap and Q-Exactive, combined with nanoLC, high-sensitivity detection of modified peptides in complex samples is achieved.
4. Data analysis and modification profiling
Professional bioinformatics tools are used to process the mass spectrometry data, identify modification types and sites, quantify modification levels, and ultimately construct histone modification profiles to provide intuitive data support for downstream functional studies.
Noberini, R. et al. FEBS J. 2022.
Service Advantages
1. Comprehensive coverage of multiple modifications
MtoZ Biolabs can systematically identify a variety of common histone modifications such as acetylation, methylation, phosphorylation, and ubiquitination, and also supports exploratory analysis of other rare modifications. With high-resolution mass spectrometry platforms and optimized data processing workflows, clients can obtain a comprehensive modification map to understand chromatin regulation mechanisms from an overall perspective.
2. Accurate localization of modification sites
The functions of histone PTMs are often closely associated with specific sites. Our histone post-translational modification identification service not only identifies the types of modifications but also pinpoints them to specific amino acid residues, helping researchers directly link modification changes with gene regulation, signal pathway activation, and other critical biological processes.
3. Quantitative and dynamic change analysis
In addition to qualitative identification, we also support semi-quantitative and quantitative analyses, enabling comparisons of histone modification dynamics under different experimental treatments or disease conditions.
4. Data interpretation and functional annotation
MtoZ Biolabs not only provides raw mass spectrometry data but also combines professional bioinformatics tools to deeply interpret modification results, including pathway enrichment analysis, functional annotation, and prediction of potential regulatory networks.
5. High customization and project support
Different research projects have varying requirements for modification types, detection depth, and analytical methods. Our histone post-translational modification identification service can be customized according to client needs. Whether it is targeted analysis of specific modifications or large-scale comparative studies, appropriate technical support and data delivery can be provided to ensure that the research results are specific and practical.
Sample Submission Suggestions
1. Acceptable sample types
MtoZ Biolabs supports various sample types for histone PTM identification, including mammalian cell lines, primary cells, clinical tissue samples (frozen or FFPE), yeast and fungal samples, and plant tissues. Clients can select the most suitable sample source according to their research goals, and we will design optimized extraction and analysis workflows based on the characteristics of different sample types.
2. Sample quantity
To ensure stability and reliability of identification, sufficient amounts of cells or tissues are required to extract adequate histones. Specific sample amounts may vary depending on experimental objectives, but it is recommended to confirm with us before submission to ensure downstream PTM identification and quantitative analysis requirements can be met.
3. Sample transportation
All samples should be transported under low-temperature conditions, preferably on dry ice, to avoid alteration of modification states. FFPE samples may be shipped at room temperature, but should be well-sealed to avoid moisture or contamination.
4. Sample storage
Cells and tissues should be snap-frozen after collection and stored at -80°C. Plant and fungal samples are also recommended to be stored in liquid nitrogen to maintain their original modification states. FFPE samples should be stored in a dry, dark environment to ensure long-term stability.
The identification of histone post-translational modifications is a key step in epigenetic research and disease mechanism exploration. With advanced mass spectrometry platforms and systematic analytical workflows, MtoZ Biolabs provides high-quality and reproducible histone post-translational modification identification service designed to advance epigenetic research, disease mechanism studies, and therapeutic discovery.
Contact us to initiate your histone modification identification project today.