Integrative Proteomics-Acetyl Analysis Service
Based on high-resolution mass spectrometry platforms and multidimensional proteomics technologies, MtoZ Biolabs has launched the integrative proteomics-acetyl analysis service achieving joint analysis of the overall proteome level and acetylation modifications. This service not only enables precise identification and quantification of acetylation modification sites but also systematically analyzes their relationships with protein abundance, functional networks, and regulatory pathways in the context of proteomics. Through specific enrichment strategies and optimized workflows, it can simultaneously output global protein expression profiles, acetylation modification maps, and functional annotations, providing reliable data support for studying the synergistic effects between acetylation and protein functional networks.
Overview
Acetylation is an important type of post-translational modification, usually occurring on lysine residues or the N-terminus of proteins, regulating protein stability, localization, and molecular interactions. Integrative Proteomics-Acetyl Analysis combines proteomics and bioinformatics approaches to systematically analyze acetylation types, sites, and abundance changes, revealing their roles in cellular function regulation and signal transduction. This service is widely applied in epigenetics, cell cycle research, metabolic regulation, and molecular biomarker screening, providing a global perspective and solid data foundation for a deeper understanding of protein acetylation.
Ree, R. et al. Experimental & Molecular Medicine, 2018.
Figure 1. Schematic Outline of N-Terminal and Lysine Protein Acetylation.
Why Integrative Proteomics-Acetyl Analysis Is Needed
Integrative acetyl proteomics can simultaneously associate protein abundance with acetylation modifications, revealing how cells achieve functional regulation at both the quantitative and modification levels. Traditional proteomics can only reflect the overall amount of proteins, while acetylation analysis reveals the role of modifications in regulating activity, localization, or interactions. Many key regulatory events occur only at the modification level, while some changes are reflected only in protein abundance. Therefore, combined analysis can more clearly distinguish between abundance-driven and modification-driven regulatory mechanisms.
1. Reducing Interpretive Bias
An increase in acetylation levels without a change in abundance indicates functional regulation rather than simple expression changes.
2. Revealing Pathway Coordination
Integrated data can show how coordinated changes in acetylation and expression affect metabolic or signaling pathways.
3. Enhancing Biomarker Research
Helps identify modification-specific biomarkers that appear earlier than abundance changes and deepens understanding of drug mechanisms.
4. Improving Research Efficiency
Focuses integrative analysis on the most biologically meaningful candidate molecules, avoiding resource waste.
Technology Platform
1. High-Resolution Mass Spectrometry Platform
Relying on advanced LC-MS/MS systems, enabling precise identification and quantification of proteomes and acetylation modifications.
2. Multiple Quantitative Strategies
Supporting Label-free, TMT, iTRAQ, DIA, and other quantitative approaches to meet different research needs.
3. Specific Enrichment Strategies for Modified Peptides
Effectively capturing low-abundance acetylated peptides through optimized enrichment methods to improve detection sensitivity and coverage.
4. Multi-Omics Integrative Analysis
Combining transcriptomics, metabolomics, and other omics data to achieve systematic interpretation of acetylation within the overall network.
Analysis Workflow
1. Sample Preparation and Protein Extraction
Perform protein extraction and preprocessing of experimental samples to ensure sample integrity and modification stability.
2. Proteolytic Digestion and Peptide Enrichment
Complete enzymatic digestion under optimized conditions and use specific enrichment methods to capture acetylated peptides.
3. Proteome and Acetylation Detection
Rely on high-resolution mass spectrometry platforms to achieve comprehensive proteome detection and acetylation modification identification and quantification.
4. Data Integration and Functional Interpretation
Combine proteomics data with acetylation modification profiles to output abundance changes, site information, and functional annotations, forming integrative analysis results.
Service Advantages
1. Global Integration
Simultaneously obtain protein abundance and acetylation modification data to construct a complete regulatory map.
2. High Sensitivity and High Accuracy
Relying on advanced mass spectrometry platforms and optimized workflows to precisely capture low-abundance modifications and ensure reliable data.
3. One-Stop Analysis
Providing systematic integrative solutions from experimental design to data interpretation.
4. Flexible Customization
Adjusting analytical strategies according to research objectives and sample characteristics to meet diverse needs.
Applications
1. Signal Pathway Research
Analyze the synergistic regulatory mechanisms of acetylation and protein expression in key signaling pathways.
2. Epigenetics Research
The integrative proteomics-acetyl analysis service can be used to reveal the role of acetylation in chromatin remodeling and gene expression regulation.
3. Drug Mechanism of Action Research
Elucidate regulatory pathways by evaluating the combined effects of drugs on protein abundance and acetylation modifications.
4. Biomarker Discovery
The integrative proteomics-acetyl analysis service can be used to identify potential diagnostic and therapeutic biomarkers.