Quantitative SUMO Proteomics Service
Quantitative SUMO proteomics aims to accurately measure the changes of SUMOylation under different physiological or pathological conditions through high-resolution mass spectrometry technology and quantitative strategies, thereby revealing the mechanisms of SUMOylation in cellular homeostasis regulation and disease occurrence. This strategy not only can identify hundreds to thousands of SUMOylated proteins but also achieve high-throughput quantification of modification levels in multi-condition comparison. It is a powerful tool for studying stress response networks, tumor signaling pathways, and drug action mechanisms.
Relying on advanced LC–MS/MS analytical platforms and systematic quantitative proteomics programs, MtoZ Biolabs provides high-quality Quantitative SUMO Proteomics Service for global research institutions and pharmaceutical companies. We use multiple quantitative modes, including SILAC, iTRAQ/TMT, Label-Free, and DIA/SWATH, to provide customers with flexible, reliable SUMO proteomics research solutions, helping promote in-depth progress in scientific research and drug development.
Services at MtoZ Biolabs
MtoZ Biolabs provides multiple customizable quantitative SUMO proteomics strategies to meet different research needs and experimental conditions:
By introducing light and heavy isotope labels during cell culture, accurate quantification of SUMOylated proteins between different treatment groups is achieved, suitable for comparative studies of cell model systems.
2. iTRAQ/TMT Multiplex Labeling Quantification
Using isotope tags for parallel analysis of multiple samples, multiple experimental groups can be quantitatively compared simultaneously, commonly used in drug intervention, stress response, and cell signaling research.
3. Label-Free Quantification
Based on MS signal intensity for peak area comparison, no prior labeling is required, suitable for natural samples or research projects with a large number of samples, with the advantages of simple operation and high flexibility.
4. DIA/SWATH Data-Independent Acquisition Quantification
By scanning all ion signals in parallel, high coverage and high reproducibility quantitative analysis of SUMOylated proteins is achieved, suitable for large-scale or time-series systematic studies.
These strategies can be flexibly selected according to sample type, research objectives, and budget, and can be combined with SUMO remnant peptide enrichment (SUMO-Remnant IAP) and high-resolution Orbitrap or Q-TOF platforms to achieve higher sensitivity and quantitative accuracy.
Analysis Workflow
The quantitative SUMO proteomics service of MtoZ Biolabs follows a standardized experimental process to ensure data reliability and reproducibility:
1. Sample Extraction and Pretreatment: Extract total proteins from cell or tissue samples and perform denaturation, reduction, and alkylation treatment.
2. SUMO Peptide Enrichment: Use SUMO-specific antibodies (SUMO-Remnant IAP) or tag systems to achieve highly specific enrichment of SUMOylated peptides.
3. Digestion and Labeling: Perform trypsin digestion and isotopic or chemical labeling according to the selected scheme.
4. LC–MS/MS Detection: Conduct multi-stage mass spectrometry analysis on high-resolution mass spectrometry systems (Orbitrap Fusion Lumos/Q Exactive HF).
5. Data Quantification and Bioinformatics Analysis: Use professional software for peak matching, quantitative comparison, significance analysis, and pathway enrichment.
6. Result Verification and Report Generation: Generate a clear data analysis report and visualization charts to support subsequent research validation.
Figure 1. Quantitative SUMO Proteomics Workflow
Service Advantages
1. Advanced Mass Spectrometry Platform
MtoZ Biolabs is equipped with multiple high-resolution mass spectrometry systems, including Thermo Fisher Orbitrap Fusion Lumos and Q Exactive HF platforms, combined with Nano-LC ultra-high-performance liquid chromatography systems to achieve excellent sensitivity and separation capability. By combining DDA and DIA modes, accurate identification and stable quantification of SUMO-modified peptides in complex samples can be achieved.
2. Multiple SUMO Enrichment Strategies
MtoZ Biolabs combines multiple enrichment strategies in quantitative SUMO proteomics, including anti-SUMO1/2/3 antibody immunoaffinity enrichment, His/FLAG tag affinity purification, and SUMO remnant peptide-specific antibody capture, achieving efficient enrichment and comprehensive coverage of low-abundance SUMOylated peptides, providing a reliable foundation for subsequent quantitative mass spectrometry.
3. Diverse Quantification Strategies
Within the Quantitative SUMO Proteomics Service offered by MtoZ Biolabs, multiple quantitative modes including SILAC, TMT, Label-Free, and DIA are available to achieve highly sensitive and reliable quantification while balancing multi-sample comparison and cost-effectiveness. All strategies have been experimentally validated to ensure reproducibility and accuracy across different sample types.
4. Professional Technical Team
Our scientists have rich experience in SUMO proteomics and post-translational modification research and can provide customized experimental design and result interpretation recommendations according to customer research goals to ensure scientific and reasonable experimental plans.
Applications
✔️ Stress Response and Cellular Homeostasis Regulation Research: Reveal the dynamic changes of SUMOylation in processes such as heat shock, oxidative stress, and DNA damage repair.
✔️ Cancer and Metabolic Disease Mechanism Research: Explore the roles of SUMO modification in transcriptional regulation, metabolic reprogramming, and drug resistance of tumor cells.
✔️ Nervous System Disease and Aging Research: Analyze the molecular mechanisms of SUMO modification in synaptic function, neurodegeneration, and aging.
✔️ Drug Target Discovery and Mechanism Verification: Reveal potential drug-regulated nodes and signaling networks through quantitative SUMO proteomics.
Sample Submission Suggestions
1. Sample Type: Cell lysates, tissue homogenates, or protein solutions are acceptable, and avoid samples containing high salt or strong detergents.
2. Protein Amount Requirement: It is recommended that each sample provide ≥1 mg of total protein, and the actual requirement depends on the quantitative scheme.
3. Sample Purity: Please ensure no visible precipitation or contaminants; if special buffer systems are used, please indicate in advance.
4. Storage and Transport: Samples should be stored at –80°C and transported on dry ice to the MtoZ Biolabs laboratory.
5. Attached Information: Please provide sample grouping information and treatment conditions to facilitate experimental design and data analysis.
MtoZ Biolabs can provide customers with detailed sample submission guidelines and standardized templates to ensure smooth experimental connection.
Deliverables
1. Complete experimental and analytical report
2. Quantitative data table of SUMOylated proteins and modification sites
3. Differential analysis results and statistical significance summary
4. Pathway enrichment and functional annotation results
5. Data visualization files
6. Original mass spectrometry data
Contact us to obtain an exclusive Quantitative SUMO Proteomics Service and explore the molecular world of protein modification together with MtoZ Biolabs.