Multi-Channel Phosphorylated Protein Analysis Service

Multi-Channel Phosphorylated Protein Analysis Service at MtoZ Biolabs focuses on comprehensive enrichment of phosphorylated peptides through multiple complementary strategies. By combining orthogonal enrichment methods, this service maximizes coverage of phosphoserine, phosphothreonine, and phosphotyrosine peptides, allowing researchers to obtain a deeper and more reliable understanding of phosphorylation signaling networks.

 

Protein phosphorylation is one of the most critical post-translational modifications regulating processes such as cell cycle progression, immune activation, and disease pathogenesis. However, phosphorylated peptides are often low in abundance and exhibit diverse chemical properties, making them difficult to capture using a single method. Multi-channel enrichment addresses this challenge by employing multiple enrichment pathways in parallel or sequentially, thereby enhancing sensitivity, specificity, and overall data confidence.

 

Analysis Workflow

MtoZ Biolabs combines advanced enrichment chemistries with high-resolution mass spectrometry to deliver robust phosphoproteome coverage. The workflow includes:

1. Sample Preparation

Proteins are extracted from cells, tissues, or biofluids and digested into peptides using optimized protocols to preserve phosphorylation states.

 

2. Multi-Channel Phosphopeptide Enrichment

Multiple enrichment strategies are applied, either in sequence or parallel, to ensure comprehensive recovery of phosphorylated peptides:

● TiO₂ Chromatography: Highly effective for capturing acidic phosphopeptides, particularly phosphoserine and phosphothreonine.

● IMAC (Immobilized Metal Affinity Chromatography): Suitable for broad phosphopeptide enrichment with high selectivity.

● Immunoprecipitation (IP): Antibody-based enrichment targeting phosphotyrosine peptides for enhanced coverage of low-abundance events.

● SCX (Strong Cation Exchange Chromatography): Provides an orthogonal separation dimension, improving overall peptide diversity and detection depth.

 

3. High-Resolution Mass Spectrometry

Enriched peptides are analyzed on Orbitrap-based LC-MS/MS platforms, ensuring high sensitivity, resolution, and reproducibility.

 

4. Data Analysis and Interpretation

Advanced bioinformatics pipelines integrate data from multiple enrichment channels, providing site-specific identification, quantitative comparisons, kinase activity prediction, and pathway annotation.

 

Figure 1. Multi-Channel Phosphorylated Protein Analysis Workflow

 

Sample Submission Suggestions

 

Note: Provide details on sample collection and handling. If you need further details, our technical support team is happy to assist and provide comprehensive guidance on sample submission.

 

Applications

Multi-Channel Phosphorylated Protein Analysis Service supports a wide range of research and development needs:

Cancer biology: Deep profiling of oncogenic signaling, including rare phosphorylation events.

Drug discovery: Evaluating kinase inhibitor effects by capturing diverse phosphorylation dynamics.

Immunology: Mapping phosphorylation cascades in immune cell activation pathways.

Cell signaling research: Exploring pathway cross-talk and phosphorylation-driven regulation.

Precision medicine: Identifying phosphorylation biomarkers for diagnosis, prognosis, and patient stratification.

 

Why Choose MtoZ Biolabs?

Partnering with MtoZ Biolabs ensures reliable and high-quality phosphoproteomics results. Key advantages include:

✅ Advanced enrichment strategies: Integration of IMAC, TiO₂, and antibody-based capture for comprehensive coverage.

✅ High throughput capability: Analysis of several thousand phosphorylation sites in a single experiment, supporting extensive and large-scale studies.

✅ Cutting-edge instrumentation: Orbitrap and timsTOF platforms ensure sensitivity, resolution, and accuracy.

✅ Expert scientific team: Decades of experience in proteomics and signaling pathway research.

✅ Actionable reporting: Clear results with visualized data and pathway-level insights tailored for research and development.

 

What Could be Included in the Report?

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment

4. Data Analysis, Preprocessing, and Estimation

5. Bioinformatics Analysis

6. Raw Data Files

 

By combining multiple enrichment strategies with high-resolution mass spectrometry, MtoZ Biolabs enables a deeper and more accurate exploration of phosphorylation networks. With high throughput capacity to analyze thousands of sites per experiment, our Multi-Channel Phosphorylated Protein Analysis Service provides robust and comprehensive datasets that accelerate discoveries in basic biology, drug development, and precision medicine, while complementing our full portfolio of proteomics solutions.