Single Cell Phosphoproteomics Service
Single Cell Phosphoproteomics Service at MtoZ Biolabs provides researchers with the ability to investigate phosphorylation dynamics at the single-cell level. This advanced service reveals heterogeneity in signaling pathways that bulk analyses often overlook, offering unprecedented insights into cellular regulation, disease mechanisms, and therapeutic responses.
Overview
Phosphorylation is a reversible post-translational modification that regulates nearly all cellular processes, including proliferation, differentiation, apoptosis, and immune activation. Traditional phosphoproteomics relies on bulk populations, masking the molecular diversity between individual cells. Single cell phosphoproteomics overcomes this limitation by enabling site-specific identification and quantification of phosphorylation events within individual cells, thereby uncovering functional variability that drives development, disease progression, and drug resistance.
Lee, S. et al. Biology. 2023.
Figure 1. Comparison of Bulk and Single Cell Analysis
Analysis Workflow
MtoZ Biolabs integrates single-cell isolation with advanced phosphoproteomic analysis to ensure reliable results from extremely limited material. The workflow includes:
1. Single-Cell Isolation
Individual cells are separated using fluorescence-activated cell sorting (FACS), microfluidic platforms, or laser capture microdissection to guarantee precision and sample integrity.
2. Protein Extraction and Digestion
Proteins from single cells are carefully processed and enzymatically digested to generate peptides suitable for downstream enrichment.
3. Phosphopeptide Enrichment
Strategies such as TiO₂, IMAC, and antibody-based immunoprecipitation are applied to selectively capture low-abundance phosphopeptides, improving coverage and sensitivity.
4. High-Resolution Mass Spectrometry
Enriched peptides are analyzed on Orbitrap-based instruments, ensuring accurate mass detection, high sensitivity, and reproducibility.
5. Data Analysis and Interpretation
Bioinformatics pipelines provide site localization, phosphorylation profiling, kinase activity prediction, and pathway enrichment, offering a detailed view of cellular heterogeneity at the single-cell level.
Why Choose MtoZ Biolabs?
✔ Integrated Single-Cell Workflows: From precise isolation (FACS, microfluidics) to downstream phosphoproteomics.
✔ Orbitrap MS Platforms: High-resolution instruments ensure sensitivity and reproducibility.
✔ Flexible Research Design: Tailored workflows support both discovery-driven and targeted studies.
✔ Comprehensive Bioinformatics: From phosphorylation site mapping to pathway enrichment and kinase activity prediction.
✔ Transparent Pricing: One-time charge with clear deliverables.
Sample Submission Suggestions
Our Single Cell Phosphoproteomics Service accepts a range of biological samples, including cultured cell lines, primary cells, fresh or frozen tissues, biofluids such as plasma or cerebrospinal fluid, and exosomes carrying phosphorylated proteins. Due to the sensitivity of phosphoproteome measurements, samples should be freshly prepared or properly frozen to preserve phosphorylation states. All submissions must be sealed to prevent contamination and degradation. For single cell studies, please provide cells suitable for isolation by FACS or microfluidics. Detailed submission guidelines are available upon request to ensure reliable results.
Applications
The Single Cell Phosphoproteomics Service supports a wide range of applications in biomedical and translational research:
Cancer Research: Identify phosphorylation-driven subpopulations that contribute to tumor heterogeneity and therapeutic resistance.
Stem Cell Biology: Map phosphorylation dynamics during differentiation, lineage commitment, and reprogramming.
Immunology: Characterize signaling heterogeneity within immune subsets such as T cells and B cells.
Drug Development: Assess kinase inhibitor effects at single-cell resolution to refine therapeutic strategies.
Precision Medicine: Profile patient-derived cells to uncover phosphorylation biomarkers for individualized treatment.
Single cell phosphoproteomics provides unprecedented insight into cellular signaling and heterogeneity. MtoZ Biolabs delivers a streamlined solution from single cell isolation to advanced mass spectrometry and bioinformatics, ensuring accurate and reproducible results. Our Single Cell Phosphoproteomics Service empowers research across basic biology, drug development, and precision medicine, while complementing our broader portfolio of proteomics and phosphoproteomics services.