Histone β-Hydroxybutyrylation Analysis Service
Based on a high-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS) platform combined with efficient peptide enrichment strategies, MtoZ Biolabs has launched histone β-hydroxybutyrylation analysis service to comprehensively detect and quantitatively analyze histone β-hydroxybutyrylation (Kbhb) modifications. This service enables accurate localization of modification sites and their abundance changes, and through integration with multidimensional bioinformatics analysis, ultimately provides data results including modification distribution patterns, quantitative differences, and functional correlations. This service helps researchers gain in-depth insights into the role of Kbhb in epigenetic regulation, metabolic balance, and disease occurrence, offering strong data support for both scientific and applied research.
Overview
Histone β-hydroxybutyrylation (Kbhb) is a type of modification in which a β-hydroxybutyryl group is attached to histone lysine residues, altering chromatin conformation and affecting transcriptional regulation of genes. Recent studies have shown that Kbhb acts as a bridge between metabolism and epigenetics, playing important roles especially in energy metabolism imbalance, diabetes, cellular stress responses, and stem cell differentiation. This service enables precise detection and quantitative analysis of the modification, assisting researchers in deeply investigating its roles in gene expression regulation, disease mechanism studies, and potential biomarker discovery, thereby providing high-quality data support for related research.
Zhou, T T. et al. Frontiers in Immunology, 2022.
Figure 1. Histone Kbhb Sites on Histones.
Analysis Workflow
Histones are extracted from cell or tissue samples to ensure the stability and integrity of the modifications.
2. Peptide Enrichment and Digestion
Specific enrichment strategies and enzymatic digestion methods are used to enrich β-hydroxybutyrylation–modified peptides.
3. LC-MS/MS Analysis
Relying on a high-resolution mass spectrometry platform, peptides are precisely analyzed and modification sites are identified.
4. Data Analysis and Annotation
Through bioinformatics analysis, results are provided on modification site distribution, abundance changes, and functional annotations.
Sample Submission Suggestions
1. Sample Type and Quantity
Note: Plasma should be collected using EDTA as an anticoagulant. Standard tissue or cell lysis buffers can be used during protein extraction.
2. Sample Transportation
Avoid repeated freeze-thaw cycles. Samples are recommended to be stored at -80°C and transported on dry ice to ensure low-temperature conditions throughout the process and prevent modification loss.
Service Advantages
1. High-Resolution Detection
Relying on advanced mass spectrometry platforms, low-abundance β-hydroxybutyrylation modification sites can be accurately captured.
2. Efficient Enrichment Strategy
Optimized peptide enrichment methods are applied to effectively improve modification identification rates and coverage.
3. Comprehensive Analysis Workflow
An integrated solution is provided from histone extraction to data analysis, ensuring reliable and reproducible results.
4. Customized Solutions
Experimental workflows are flexibly adjusted according to sample characteristics and research objectives to meet diverse scientific needs.
Applications
1. Metabolic Regulation Research
By analyzing β-hydroxybutyrylation modifications, their association with cellular energy metabolism states can be explored.
2. Epigenetics Research
Histone β-hydroxybutyrylation analysis service can be used to reveal the role of Kbhb in chromatin structural changes and gene expression regulation.
3. Potential Biomarker Discovery
Systematic identification of modification patterns associated with specific physiological states helps in potential biomarker screening and application.
4. Cell Cycle and Developmental Research
Histone β-hydroxybutyrylation analysis service can be applied to monitor its dynamic changes during cell division, differentiation, and stem cell development.
FAQ
Q1: Kbhb and 2-Hydroxyisobutyrylation (Khib) Have the Same Mass. How Can They Be Distinguished?
A1: They are isomers and isobaric, so conventional mass differences cannot separate them. We employ specific immunoenrichment and optimized liquid chromatography separation to enhance retention time differences, combined with fragment ion spectral features and negative/positive control validation to reduce misidentification risk. If necessary, targeted validation strategies can be applied to increase confidence.
Q2: How Should Fragmentation Modes Be Selected to Improve Site Localization Accuracy?
A2: High-resolution HCD is prioritized, combined with stepped-energy strategies to maximize information acquisition. Depending on project requirements, ETD/EThcD can be introduced to better preserve modification side chain information and improve site localization probability. Ion matching, ion series continuity, and characteristic ions are integrated to strengthen the evidence chain.