SUMOylated Peptide Enrichment Service
Post-translational modifications (PTMs) are essential mechanisms regulating cellular processes, signal transduction, and protein functionality. Among these, SUMOylation is a critical PTM type. SUMO (Small Ubiquitin-like Modifier) refers to a family of small proteins covalently attached to lysine residues of target proteins, thereby modulating their stability, subcellular localization, interactions, and transcriptional activity. SUMOylation plays a central role in biological processes such as cell cycle regulation, DNA damage repair, and stress response. Therefore, precise identification of SUMOylation sites is crucial for understanding protein regulation and cellular signaling networks in proteomics studies.
However, SUMOylated proteins are typically of low abundance in cells, and the modification is both reversible and dynamic. Thus, peptide enrichment prior to mass spectrometry analysis is particularly important to selectively capture SUMOylated peptides, enhance detection sensitivity, and improve site identification accuracy. Leveraging advanced proteomics platforms and extensive experience in PTM research, MtoZ Biolabs offers professional SUMOylated peptide enrichment service to support researchers in comprehensive SUMOylation analysis.
Services at MtoZ Biolabs
At MtoZ Biolabs, we provide multiple SUMOylated peptide enrichment strategies tailored to diverse research needs:
1. Anti-SUMO Antibody Immunoprecipitation (IP)
Utilizes SUMO1- and SUMO2/3-specific antibodies to selectively capture SUMOylated proteins or peptides. This approach is suitable for both protein-level and peptide-level SUMOylation studies.
2. Tag-Based Affinity Enrichment
Incorporates affinity tags such as His-tag or FLAG-tag at the SUMO terminus, followed by affinity purification using specific antibodies or resins. This method is ideal for low-abundance samples or studies requiring high specificity.
3. Peptide-Level Enrichment After Enzymatic Digestion
Proteins are digested (e.g., with trypsin), and antibodies targeting the characteristic SUMO remnant peptides are used to selectively enrich SUMOylated peptides. This approach is highly compatible with mass spectrometry for precise site identification.
4. Multidimensional Chromatography Combination
Combines ion exchange (IEC) and reversed-phase HPLC with antibody-based or affinity-based enrichment to improve sample purity and detection sensitivity, optimizing complex sample analyses.
MtoZ Biolabs ensures the acquisition of high-quality SUMOylated peptide samples, providing a reliable foundation for subsequent qualitative and quantitative mass spectrometry analyses.
Analysis Workflow
1. Sample Preparation
Collect cell or tissue samples, extract and quantify proteins, ensuring sample quality and consistency.
2. Protein Digestion
Digest protein samples (e.g., using trypsin) to generate analyzable peptides.
3. SUMOylated Peptide Enrichment
Select the appropriate enrichment strategy based on experimental objectives to specifically capture SUMOylated peptides.
4. Mass Spectrometry Analysis
Analyze enriched peptides using LC-MS/MS to obtain sequence and modification site information.
5. Data Processing and Reporting
Perform database searches, modification site identification, and quantification, followed by the generation of a structured analysis report to support downstream research.
Service Advantages
1. Multiple Enrichment Strategies
Our SUMOylated peptide enrichment service provides diverse enrichment methods, including antibody-based, tag-based, and peptide-level enrichment. These approaches can be flexibly combined according to sample type, abundance, and research goals to achieve high specificity and recovery rates.
2. Advanced Mass Spectrometry Platform
Equipped with state-of-the-art LC-MS/MS systems and robust data processing workflows, we accurately identify SUMOylated peptides and their modification sites, ensuring data reliability and high analytical quality.
3. Expert Scientific Support
Our experienced proteomics team provides professional guidance in experimental design, sample optimization, and data interpretation, ensuring scientifically sound solutions and maximizing research value.
4. One-Time Charge
Our pricing is transparent, with no hidden fees or additional costs.
Applications
1. Protein Function Analysis: Explore how SUMOylation regulates protein stability, localization, and interaction networks.
2. Cell Signaling Research: Investigate the role of SUMOylation in stress response, cell cycle progression, and DNA repair.
3. Proteomics Studies: Combine with quantitative proteomics to reveal SUMOylation dynamics and regulatory networks.
4. Drug Discovery and Target Identification: Identify SUMOylation sites to support target validation and drug development.
MtoZ Biolabs’ SUMOylated peptide enrichment service is suitable for basic research, translational studies, and drug discovery applications.
Sample Submission Suggestions
1. Sample Type: Cell lysate, tissue homogenate, or purified protein.
2. Protein Concentration: Recommended ≥1 mg/mL to ensure enrichment efficiency.
3. Buffer Conditions: Use non-reducing, low-salt buffers and avoid strong detergents.
4. Sample Amount: Depends on the chosen strategy and sample type; please consult our technical team for optimal quantity.
5. Sample Transport: Maintain low temperature (on ice or dry ice) to preserve protein and modification stability.
MtoZ Biolabs provides detailed sample submission guidelines and can offer customized preparation plans based on client requirements.
As a critical post-translational modification, SUMOylation plays an essential role in cellular regulation and signaling network studies. Relying on advanced instrumentation, expert teams, and flexible enrichment strategies, MtoZ Biolabs delivers high-quality SUMOylated peptide enrichment service, empowering researchers to obtain reliable results in SUMOylation proteomics.
Contact MtoZ Biolabs today to learn how our SUMOylated peptide enrichment solutions can support your research.