Methylation Proteomics Service
Protein methylation is a key post-translational modification that primarily occurs on lysine and arginine residues. It regulates gene expression, chromatin structure, signal transduction, and RNA processing, and is mediated by methyltransferases using S-adenosylmethionine (SAM) as the donor. Depending on the enzyme and context, residues may undergo mono-, di-, or tri-methylation, each with distinct functional outcomes.
Abnormal methylation profiles are closely associated with cancer, neurodegeneration, immune disorders, and aging. By combining selective enrichment strategies with high-resolution mass spectrometry, MtoZ Biolabs provides Methylation Proteomics Service for global profiling and quantitative analysis of methylated proteins, delivering accurate, reproducible, and publication-ready datasets that support both basic and translational research.
Biggar, K. K. et al. Nat. Rev.. Mol. Cell Biol. 2014.
Figure 1. Lys and Arg Methylation
Services at MtoZ Biolabs
MtoZ Biolabs provides a comprehensive range of methylation proteomics services tailored to diverse research needs:
● Methylated Peptide Enrichment Service
Optimized affinity-based approaches for the selective capture of methylated peptides, enhancing detection of low-abundance modifications.
● Methylation Site Identification Service
High-confidence localization of methylation sites on lysine and arginine residues, covering mono-, di-, and tri-methylation states.
● Quantitative Methyl-proteomics Service
Flexible strategies including Label-Free, TMT/iTRAQ, and DIA workflows for dynamic profiling of methylation across conditions.
● Methyl-proteomics Data Analysis Service
Advanced bioinformatics pipelines for site mapping, quantitative comparisons, pathway enrichment, and protein interaction network modeling.
Figure 2. Workflow of Methylation Proteomics
Why Choose MtoZ Biolabs?
✅ Specialized enrichment strategies: High-affinity antibodies for lysine and arginine methylation ensure comprehensive coverage.
✅ High-throughput capability: Detection of thousands of methylation sites in a single experiment.
✅ Advanced instrumentation: Orbitrap and timsTOF platforms guarantee accuracy and sensitivity.
✅ Expert scientific team: Strong expertise in PTM proteomics and epigenetic regulation.
✅ Actionable reporting: Clear, publication-ready datasets with functional interpretation to support decision-making.
✅ One-time charge: Our pricing is transparent, no hidden fees or additional costs.
Sample Submission Suggestions
Note: Provide details on sample collection and handling. If you need further details, our technical support team is happy to assist and provide comprehensive guidance on sample submission.
Applications
Methylation proteomics offers powerful insights into diverse biological and clinical research areas:
● Cancer biology: Characterizing methylation-regulated oncogenes, tumor suppressors, and chromatin modifiers.
● Epigenetics and gene regulation: Mapping histone methylation in transcriptional control and chromatin remodeling.
● Signal transduction: Defining methylation-driven modulation of kinases, receptors, and transcription factors.
● Neurobiology: Investigating methylation-dependent regulation of synaptic plasticity and neurodegenerative pathways.
● Drug discovery: Assessing methyltransferase and demethylase targets and evaluating small-molecule inhibitors.
FAQ
Q1: Can mono-, di-, and tri-methylation states be distinguished confidently?
By combining high-specificity enrichment with advanced mass spectrometry, MtoZ Biolabs delivers robust and high-throughput results through our Methylation Proteomics Service, enabling large-scale analysis of methylation dynamics. Beyond methylation, we offer a comprehensive portfolio of post-translational modification proteomics services, providing researchers with integrated solutions to accelerate discoveries in biology, drug development, and precision medicine.