Amino Acid-derived Modifications Analysis Service
As a professional CRO company specializing in proteomics and multi-omics detection, MtoZ Biolabs provides systematic amino acid-derived modifications analysis service with high-resolution mass spectrometry platforms, well-established sample preparation systems, and an experienced analytical team. Our service covers multiple modification types and integrates complete data interpretation and functional annotation, aiming to provide reliable and comprehensive solutions for scientific and industrial clients to advance life sciences and translational medicine.
What is an Amino acid-derived modification
Amino acid-derived modifications are an important and special class of protein modifications. The donors or modification groups of these modifications usually originate directly from amino acids or their metabolic derivatives, which can introduce new chemical groups at the protein level and alter the charge, conformation, and stability of proteins. For example, acetylation and methylation are derived from acetyl-CoA and S-adenosylmethionine (SAM), while hydroxylation, citrullination, and γ-carboxylation are closely related to amino acid metabolism.
Amino acid-derived modifications play critical roles in cell signaling, metabolic adaptation, chromatin regulation, and disease development. Abnormal levels or distribution of these modifications are often closely associated with cancer, cardiovascular diseases, neurodegenerative disorders, and immune-related diseases. Therefore, their detection and analysis are essential for elucidating molecular mechanisms, discovering disease biomarkers, and advancing drug development.
Services at MtoZ Biolabs
MtoZ Biolabs provides a systematic amino acid-derived modifications analysis service that covers the complete process from sample preparation and mass spectrometry detection to data interpretation, ensuring research depth and reliability. Our service includes:
1. Target Protein Amino Acid-Derived Modifications Analysis
MtoZ Biolabs provides Target Protein Amino Acid-Derived Modifications Analysis to detect a wide range of amino acid-derived modifications on specific target proteins, including but not limited to carbonylation, nitration, chlorination, hydroxylation, amination, deamidation, and various oxidation-derived side-chain modifications. Using antibody-based enrichment, chemical labeling approaches such as DNPH derivatization for carbonylation, and selective affinity capture methods, we enhance the detection of modification-associated peptides.
Coupled with high-resolution LC-MS/MS and multiple fragmentation modes including CID, HCD, ETD, and EThcD, this service can accurately determine whether modifications occur, pinpoint modification sites, identify modification types, and quantify abundance changes. It effectively addresses analytical needs such as modification site confirmation, condition-dependent modification comparison, and mutation effect evaluation, and provides key outputs including modification site lists, quantitative results, and supporting MS/MS evidence.
2. Amino Acid-Derived Modifications Proteomics Analysis
MtoZ Biolabs Amino Acid-Derived Modifications Proteomics Analysis enables systematic identification and quantification of amino acid-derived modifications at the proteome level, supporting global modification profiling, comparison of modification differences across conditions, and analysis of dynamic modification changes. Through chemical labeling enrichment such as DNPH derivatization for carbonylation, specific antibody-based capture, and high-throughput LC-MS/MS, we can identify large numbers of modified proteins, modified peptides, and precise modification sites.
With quantitative approaches including TMT and Label-free strategies, we generate cross-sample modification abundance matrices, site-level variation trends, and statistical analysis outputs, providing clients with comprehensive proteome-wide datasets on amino acid-derived modifications.
Analysis Workflow
1. Sample preparation: Clients provide cell, tissue, or body fluid samples for protein extraction and pretreatment.
2. Modification enrichment: Depending on research objectives, specific chemical or affinity methods are used to selectively enrich target modified peptides.
3. Mass spectrometry detection: High-resolution LC-MS/MS systems are applied to acquire raw data.
4. Data processing: Quality control, peptide identification, and modification site localization are performed.
5. Bioinformatics analysis: Includes modification site annotation, functional enrichment, and pathway analysis to generate visualized results.
Service Advantages
1. Advanced Platform
MtoZ Biolabs is equipped with multiple high-resolution mass spectrometry systems, including Orbitrap Fusion Lumos and Q Exactive HF, combined with Nano-LC systems. This enables highly sensitive detection of low-abundance modifications and ensures reliable capture of modifications in complex samples.
2. Experienced Team
Our team has long been engaged in post-translational modification research and is familiar with the detection of various amino acid-derived modifications. We are able to provide scientifically sound detection strategies based on different experimental needs, ensuring feasibility and reliability.
3. One-Time Charge
Our pricing is transparent, with no hidden fees or additional costs.
Applications
✔️ Basic research: Reveal the regulatory mechanisms of amino acid-derived modifications on protein function and signaling networks.
✔️ Disease research: Study the relationship between abnormal modifications and cancer, metabolic disorders, cardiovascular diseases, and immune diseases.
✔️ Drug development: Monitor the impact of candidate drugs on modification levels to support target discovery and efficacy validation.
✔️ Biopharmaceuticals: Used in protein drug development for modification state characterization and functional verification.
Sample Submission Suggestions
| Item | Details |
| Sample types | Cells, tissues, serum/plasma, etc., with no obvious degradation |
| Sample amount | Provide sufficient total protein according to experimental needs, recommended ≥100 μg |
| Storage and transport | Samples should be stored at low temperature and transported on dry ice, avoiding repeated freeze-thaw cycles |
| Other instructions | Detailed sample submission guidelines can be provided by MtoZ Biolabs |
Deliverables
1. Detailed report of sample processing and experimental workflow
2. Raw mass spectrometry data files (e.g., .raw/.mzML)
3. Modification site identification and quantitative results
4. Visualized charts of functional enrichment and pathway analysis
5. Standardized final analysis report, supporting further research and publication
Relying on advanced mass spectrometry platforms and a professional research team, MtoZ Biolabs provides a systematic amino acid-derived modifications analysis service. From sample preparation and modification detection to data interpretation, we are committed to delivering reliable research support for scientific and industrial clients. Contact us to advance proteomics and life science research together.