DNA/RNA Modification Analysis Service
Chemical modifications of DNA and RNA represent essential molecular mechanisms that regulate gene expression and maintain genomic stability. DNA modifications such as 5-methylcytosine and 5-hydroxymethylcytosine, together with RNA modifications including N6-methyladenosine, m5C, and pseudouridine, form a complex epigenetic regulatory network that influences transcription, splicing, translation, and degradation. These modifications play vital roles in cellular differentiation, embryonic development, stress response, and disease progression. Systematic analysis of DNA and RNA modifications provides valuable insights into dynamic gene regulation and helps reveal the fundamental principles of biological processes.
With the rapid advancement of high-resolution mass spectrometry, liquid chromatography-tandem mass spectrometry (LC-MS/MS) has become the core analytical approach for detecting and quantifying DNA and RNA modifications. This technique enables highly sensitive identification of various modified nucleosides and supports both global quantification and site-specific characterization. Relying on advanced mass spectrometry platforms and extensive expertise in nucleic acid chemistry, MtoZ Biolabs provides a comprehensive and reliable DNA/RNA Modification Analysis Service to help researchers explore epigenetic regulatory mechanisms in depth.
Services at MtoZ Biolabs
MtoZ Biolabs offers DNA/RNA Modification Analysis Service based on high-resolution LC-MS platforms combined with optimized sample preparation and quantitative strategies. This service enables accurate identification and quantification of multiple types of nucleic acid modifications. Our analysis covers a wide range of DNA and RNA modifications, including but not limited to:
- mRNA Modification LC-MS Analysis Service
- tRNA Modification LC-MS Analysis Service
- m6A Modification LC-MS Analysis Service
We also provide detection of other modifications such as 5-mC, 5-hmC, m1A, m5C, m7G, pseudouridine, and m2G. If you are interested in our services, please feel free to contact us for more information.
Analysis Workflow
1. Sample Preparation and Quality Control: Extract high-purity DNA or RNA and assess concentration and integrity
2. Enzymatic Digestion: Completely digest nucleic acids into single nucleosides or partial oligonucleotides
3. Liquid Chromatography Separation: Use reversed-phase or hydrophilic interaction chromatography for efficient separation of modified nucleosides
4. Mass Spectrometry Detection: Acquire precursor and fragment ion data using high-resolution LC-MS/MS systems
5. Data Processing and Quantification: Identify and quantify modifications based on characteristic ion matching and calibration with reference standards
6. Report Generation and Modification Profiling: Provide visualized modification distribution reports including statistical charts and data summaries
Ogawa, A. et al. STAR Protoc. 2021.
Figure 1. DNA/RNA Modification Analysis Workflow
Service Advantages
1. High Sensitivity
MtoZ Biolabs utilizes advanced high-resolution mass spectrometry platforms such as Orbitrap and Triple Quadrupole systems to ensure accurate detection of low-abundance modified nucleosides. Multi-stage fragmentation analysis enables differentiation of structurally similar isomers, ensuring high data reliability and reproducibility.
2. Broad Applicability
Our DNA/RNA modification analysis service covers multiple nucleic acid types, including DNA, mRNA, tRNA, rRNA, and non-coding RNAs. Customized sample preparation strategies are designed according to molecular characteristics, maintaining the stability of modifications and preserving native molecular states during analysis.
3. Multi-Level Analytical Strategy
Depending on research objectives, MtoZ Biolabs provides both nucleoside-level quantification and site-specific analysis. Nucleoside-level quantification is ideal for global modification profiling, while site-specific analysis allows detailed exploration of local regulatory effects, achieving both qualitative and quantitative insights.
4. One-Time Charge
Our pricing is transparent, with no hidden fees or additional costs.
Applications
1. Epigenetics Research: Investigating the roles of DNA and RNA modifications in gene expression regulation and cell differentiation
2. Disease Mechanism Studies: Exploring associations between abnormal modification patterns and diseases such as cancer, metabolic disorders, and neurological conditions
3. Pharmacological and Toxicological Research: Assessing the impact of drug candidates on nucleic acid modification patterns
4. Developmental and Environmental Response Studies: Examining dynamic modification changes during embryonic development and stress adaptation
5. Transcriptional and Translational Regulation: Understanding how RNA modifications affect splicing, transport, and translational efficiency
Deliverables
1. A complete experimental report detailing analytical procedures and data summaries
2. Quantitative tables listing all detected modified nucleosides and their abundances
3. Modification distribution profiles and statistical analysis results
4. Raw and processed mass spectrometry data files
The study of DNA and RNA modifications has become a crucial frontier for understanding gene regulation and disease mechanisms. With a mature LC-MS/MS platform and expertise in nucleic acid chemistry, MtoZ Biolabs provides high-quality DNA/RNA Modification Analysis Service to support researchers worldwide. From sample preparation to data interpretation, our professional team delivers comprehensive analytical solutions. Contact MtoZ Biolabs today to begin your nucleic acid modification analysis journey.