Histone Post-Translational Modification Analysis Service
Histone post-translational modifications (Histone PTMs) refer to various chemical modifications that occur on histone tails or core domains, including acetylation, methylation, phosphorylation, ubiquitination, etc. These modifications play a key role in regulating chromatin structure and gene transcription activity and are regarded as an important part of epigenetics. Histone PTMs can determine gene activation or silencing and have profound effects on cell differentiation, signal transduction, DNA repair, and disease development.
Figure 1. Post-Translational Modifications of Histone Amino Terminus
With the rapid development of mass spectrometry technology, MS-Based histone PTM analysis has become the core tool in this field. Mass spectrometry can achieve high-resolution identification of modification types and sites, and combined with quantitative strategies, reveal dynamic changes of modifications under different conditions. This provides strong data support for elucidating chromatin regulatory mechanisms, discovering epigenetic markers, and promoting drug development.
As a CRO company focusing on proteomics and mass spectrometry analysis, MtoZ Biolabs provides systematic Histone Post-Translational Modification Analysis Service with advanced mass spectrometry platforms and extensive experience. Our service covers histone isolation and enrichment, PTM identification, PTM quantitative analysis, and data interpretation, aiming to provide reliable and comprehensive solutions for global scientific and industrial clients, supporting epigenetics and life science research.
Services at MtoZ Biolabs
Analysis Workflow
1. Sample preparation: Clients submit qualified cell or tissue samples for protein extraction and quality control.
2. Histone isolation and enrichment: Selective separation and enrichment of target modifications using multiple strategies.
3. Mass spectrometry detection: High-resolution LC-MS/MS is used to acquire raw data.
4. Data processing: Including quality control, peptide identification, and modification site localization.
5. Bioinformatics interpretation: Conduct quantitative analysis, motif study, and functional enrichment, providing visualized results.
Noberini, R. et al. FEBS J. 2022.
Figure 2. Workflow for Histone Post-Translational Modification Analysis
Service Advantages
1. Advanced Platform
MtoZ Biolabs is equipped with multiple high-resolution mass spectrometry systems, including Thermo Fisher Orbitrap Fusion Lumos and Q Exactive HF, combined with Nano-LC systems, enabling high-sensitivity detection of complex modifications.
2. Experienced Team
Our team has long been dedicated to proteomics and PTM research, accumulating extensive project experience and being familiar with the handling of different sample types. Through proper experimental design and execution, experimental error can be effectively reduced and data reliability improved.
3. One-Time Charge
Our pricing is transparent, with no hidden fees or additional costs.
Applications
✅ Basic research: Reveal the role of histone modifications in gene regulation and chromatin structure.
✅ Disease research: Study the relationship between abnormal histone modifications and cancer, metabolic diseases, and neurodegenerative diseases.
✅ Drug development: Evaluate the impact of small molecules or epigenetic drugs on modification levels, assisting target discovery and validation.
✅ Biopharmaceuticals: Used for structural characterization and functional verification in the development of antibodies and protein drugs.
Sample Submission Suggestions
1. Sample types: Cells, tissues, serum/plasma, etc., with no obvious degradation.
2. Sample amount: Provide sufficient total protein according to experimental needs, recommended ≥100 μg.
3. Storage and transport: Samples should be stored at low temperature and transported on dry ice, avoiding repeated freeze-thaw cycles.
4. Other instructions: Detailed submission guidelines can be obtained from MtoZ Biolabs.
Deliverables
1. Detailed report of sample processing and experimental workflow
2. Raw mass spectrometry data files
3. Modification site identification and quantitative results
4. Visualized charts of functional enrichment and pathway analysis
5. Standardized final analysis report (for further research and publication)
Relying on advanced mass spectrometry platforms and a professional team, MtoZ Biolabs provides systematic Histone Post-Translational Modification Analysis Service for scientific and industrial clients. From histone isolation and enrichment to modification identification and data interpretation, we are committed to providing reliable support for your research. Contact us to advance proteomics and life science exploration together.