Glycoproteomics Analysis Service

Glycoproteomics, the large-scale study of glycosylated proteins, aims to understand how carbohydrate modifications regulate protein function and cellular behavior. Glycosylation occurs primarily at asparagine (N-linked) or serine/threonine (O-linked) residues, mediated by complex enzymatic pathways within the endoplasmic reticulum and Golgi apparatus. Abnormal glycosylation is associated with cancer progression, autoimmune disorders, infectious diseases, and neurodegenerative conditions. Therefore, glycoproteomics has become an indispensable tool in systems biology, precision medicine, and therapeutic glycoprotein characterization.

MtoZ Biolabs offers a comprehensive Glycoproteomics Analysis Service that combines state-of-the-art liquid chromatography–mass spectrometry (LC-MS/MS), advanced glycopeptide enrichment methods, and powerful bioinformatics to provide high-resolution identification profiling of glycoproteins and glycosylation sites. Our glycoproteomics analytical platforms support precise glycan structure characterization, site-specific glycosylation mapping, and quantitative analysis across biological conditions. The service enables researchers to explore glycosylation dynamics, discover biomarkers, and reveal regulatory mechanisms underlying complex biological processes.

Services at MtoZ Biolabs

1. What We Offer

💠Comprehensive Glycosylation Profiling

Global analysis of glycoproteomes across serum, tissues, cultured cells, and recombinant proteins, providing a full landscape of glycan heterogeneity and site occupancy.

💠Intact Glycopeptide Analysis

Preservation of glycan–peptide linkages for simultaneous interpretation of glycan structures and peptide backbones through high-resolution MS and HCD/EThcD fragmentation.

💠Quantitative Glycoproteomics

Label-free, TMT/iTRAQ, or DIA-based workflows for dynamic comparison of glycosylation across biological conditions, disease states, or treatment responses.

💠Bioinformatics and Data Integration

Comprehensive identification, site annotation, glycan composition analysis, and pathway enrichment using specialized bioinformatics tools for functional interpretation.

2. Our Specialized Services

🧩Protein N-Glycosylation Profiling Service

Identification and quantification of N-linked glycosylation sites using enzymatic deglycosylation (e.g., PNGase F), followed by LC-MS/MS detection for structural and occupancy analysis.

🧩Protein O-Glycosylation Profiling Service

Analysis of mucin-type and other O-linked glycans through β-elimination and lectin affinity enrichment, revealing site-specific O-glycosylation diversity and biological implications.

🧩Viral Glycoproteomics Service

Characterization of viral glycoproteins such as envelope or spike proteins to define glycosylation signatures involved in viral entry, immune evasion, and vaccine development.

🧩Venom Glycoproteomics Service

Systematic profiling of glycoproteins derived from animal, insect, or marine venoms to explore how glycosylation modulates toxin stability, host specificity, and pharmacological activity.

Analysis Workflow

1. Sample Preparation: Proteins are extracted, quantified, reduced, alkylated, and digested into peptides under optimized conditions that preserve glycosylation integrity.

2. Glycopeptide Enrichment: Lectin affinity, HILIC, or hydrazide-based chemistries are used to selectively enrich glycopeptides.

3. LC-MS/MS Analysis: High-resolution mass spectrometric detection and fragmentation are performed to identify glycan compositions and modification sites.

4. Data Processing: Glycopeptide spectra are interpreted using advanced search engines and customized glycan databases.

5. Bioinformatics Interpretation: Quantitative and structural data are integrated with biological pathway analysis to reveal functional significance.

Sample Submission Suggestions

1. Sample Types: Serum, plasma, cell lysates, tissue extracts, or purified glycoproteins.

2. Recommended Amount: At least 100 µg of total protein for LC-MS/MS analysis.

3. Preservation: Samples should be frozen at –80°C and shipped on dry ice to maintain stability.

4. Pre-consultation: For special sample types or low-yield materials, please contact us before preparation.

Why Choose MtoZ Biolabs?

✅Advanced Platform: Cutting-edge LC-MS/MS systems, integrated with optimized glycopeptide enrichment technologies, ensure high sensitivity and precision.

✅High Data Quality: AI-driven bioinformatics workflows provide accurate glycan assignment and reliable site-level quantification.

✅Comprehensive Workflow: From sample preparation to final data interpretation, we offer end-to-end glycoproteomic analysis.

✅Expert Scientific Team: Experienced specialists in proteomics, glycomics, and mass spectrometry deliver professional technical support.

✅Customizable Solutions: Flexible experimental designs tailored to diverse research objectives and sample types.

Applications

1. Disease Mechanism Research

Reveals how glycosylation changes contribute to cancer, autoimmune disorders, and neurodegenerative diseases.

2. Drug Development

Supports biopharmaceutical research by analyzing glycan structures that influence drug stability, efficacy, and immunogenicity.

3. Biomarker Discovery

Identifies glycoprotein-based biomarkers for disease diagnosis and prognosis.

4. Neurological and Developmental Research

Uncovers glycosylation's impact on neuronal function, differentiation, and signaling pathways.