Protein Microarray-Based PTM Analysis Service
Protein microarrays represent one of the most powerful high-throughput tools in modern proteomics. By immobilizing thousands of proteins or antibodies on solid supports such as glass slides or membranes, protein microarrays enable parallel investigation of protein expression, binding events, and post-translational modifications. Post-translational modifications include phosphorylation, acetylation, ubiquitination, glycosylation, and many others that regulate protein activity, stability, and cellular localization. Studying PTMs is essential for understanding dynamic signaling pathways, disease mechanisms, and potential therapeutic targets.
Citalan-Madrid, AF. et al. Hematology. 2019.
Figure 1. Schematic Overview of Protein Microarrays
Protein microarray-based PTM analysis offers a high-throughput solution that allows researchers to analyze hundreds or even thousands of proteins simultaneously with minimal sample input. It provides a panoramic view of modification states across signaling networks and cellular pathways.
Del Rincón, SV. et al. PLoS One. 2010.
Figure 2. Protein Microarray-Based PTM Analysis
MtoZ Biolabs has established a specialized protein microarray-based PTM analysis service that integrates advanced microarray technology, high-quality antibodies, and rigorous bioinformatics pipelines. Our service is designed to provide researchers with accurate, reproducible, and biologically meaningful insights into protein modifications across diverse biological systems.
Services at MtoZ Biolabs
MtoZ Biolabs provides a comprehensive protein microarray-based PTM analysis service that integrates experimental expertise with advanced bioinformatics. Our services cover the entire workflow from sample preparation to biological interpretation, ensuring reliable and actionable results for your research.
1. PTM Profiling Across Protein Microarrays
We perform systematic detection of phosphorylation, acetylation, ubiquitination, glycosylation, SUMOylation, and histone modifications across large sets of proteins simultaneously. This allows for a panoramic overview of modification dynamics under different experimental conditions.
2. High-Throughput Screening of PTM Enzymes
Our protein microarray-based PTM analysis service supports in vitro assays using protein microarrays to investigate the activity of kinases, phosphatases, acetyltransferases, and other PTM-related enzymes. This is particularly valuable for identifying enzyme-substrate relationships and mapping signaling cascades.
3. Reverse Phase Protein Array (RPPA) Analysis
We provide RPPA services to quantify protein expression and PTM levels from limited biological samples such as patient biopsies or clinical serum. RPPA enables large-scale comparative studies across hundreds of samples with high reproducibility.
4. Quantitative Comparison of PTMs
Our protein microarray-based PTM analysis service includes rigorous quantification of PTM levels between different sample groups, treatment conditions, or disease states. This comparative analysis highlights differential modifications and identifies biomarkers of interest.
5. Bioinformatics and Pathway Analysis
We integrate robust bioinformatics workflows to convert microarray signals into biologically meaningful insights. Services include signal normalization, statistical modeling, pathway enrichment, protein interaction network construction, and visualization of PTM landscapes.
6. Customized Array Design
We offer customized array formats tailored to project-specific needs, including pathway-focused panels, disease-oriented panels, or fully customized protein/antibody arrays. This flexibility ensures that your research questions are addressed with maximum efficiency.
Why Choose MtoZ Biolabs
1. One-stop Service: From experimental design to bioinformatics interpretation, we support you throughout the entire workflow.
2. Advanced Platforms: Our access to state-of-the-art protein microarray technologies and sensitive signal detection systems ensures robust performance.
4. Data Reliability: Rigorous quality control is applied at every stage of analysis to secure reproducible results.
Applications
1. Cancer research
- Profiling phosphorylation signaling pathways in tumor progression.
- Identifying aberrant acetylation or ubiquitination signatures.
- Discovering biomarkers for early diagnosis and therapeutic response.
2. Drug discovery and development
- Screening compounds for their impact on kinase or phosphatase activity.
- Assessing drug-induced modulation of PTM signaling pathways.
- Identifying off-target effects of small molecules or biologics.
3. Neurological disease studies
- Investigating abnormal phosphorylation in neurodegeneration.
- Mapping ubiquitination networks related to protein aggregation.
4. Epigenetic and histone modification research
- Systematic mapping of histone PTMs across cell states.
- Linking acetylation and methylation patterns to transcriptional activity.
5. Clinical translational research
- Analyzing PTM signatures in patient samples such as serum or biopsies.
- Developing personalized medicine strategies based on modification profiles.
Sample Submission Suggestions
1. Sample types: Accepted materials include cultured cells, tissues, body fluids, or serum.
2. Quantity: Typically 50 to 200 micrograms of protein lysate per array, though requirements may vary based on the project.
3. Storage conditions: Samples should be snap frozen in liquid nitrogen and stored at minus 80 degrees Celsius.
4. Transport: Ship samples on dry ice to maintain integrity.
5. Additional notes: Please indicate any tags, fusion proteins, or modifications that may affect antibody binding.
We encourage clients to consult our technical team for tailored guidance based on sample type and study design.
Deliverables
1. Experimental details
2. Raw and processed data
3. Statistical analysis
4. Pathway enrichment analysis
5. Network analysis
6. High-resolution figures
7. Final report
All deliverables are designed to be publication-ready and suitable for regulatory submission if required.
By partnering with MtoZ Biolabs, you gain access to a dedicated team and state-of-the-art resources to accelerate your discoveries in cancer biology, neuroscience, drug development, and clinical research.
Contact us today to discuss your project and explore how our protein microarray-based PTM analysis service can advance your research goals.