Phosphopeptide Enrichment Service
Protein phosphorylation regulates key biological processes, yet phosphorylated peptides are usually low in abundance and often masked by non-phosphorylated peptides, making direct detection difficult. Phosphopeptide enrichment is therefore essential for phosphoproteomics studies, improving sensitivity and accuracy of phosphorylation site identification.
Common challenges include low recovery, residue bias, background interference, and poor reproducibility. MtoZ Biolabs offers Phosphopeptide Enrichment Service using titanium dioxide (TiO₂) chromatography, immobilized metal affinity chromatography (IMAC), strong cation exchange chromatography (SCX), and immunoprecipitation (IP) methods to overcome these issues, ensuring broad coverage, high specificity, and consistent results. We also provide full downstream phosphoproteomics solutions to support biomedical, pharmaceutical, and agricultural research.
Services at MtoZ Biolabs
MtoZ Biolabs provides multiple enrichment options tailored to different research needs:
● TiO₂-Based Enrichment
Titanium dioxide beads bind phosphopeptides through strong affinity interactions between phosphate groups and metal oxides. This method is widely used for large-scale analysis of serine and threonine phosphorylation, providing high specificity and broad coverage in complex samples.
● IMAC (Immobilized Metal Affinity Chromatography)
IMAC uses chelated metal ions (commonly Fe³⁺ or Ga³⁺) to selectively capture phosphopeptides. It is particularly suitable for detecting multi-phosphorylated peptides and complements TiO₂-based methods by improving recovery of certain peptide classes.
● SCX (Strong Cation Exchange Chromatography)
SCX separates peptides based on charge state, where phosphopeptides typically display lower positive charges due to the presence of phosphate groups. This orthogonal approach is often combined with TiO₂ or IMAC to increase depth and coverage, especially in highly complex samples.
● Immunoprecipitation (IP)
Immunoprecipitation uses phosphorylation-specific antibodies, such as those against phosphotyrosine, to selectively isolate subsets of phosphopeptides. This approach is particularly powerful for capturing low-abundance or site-specific phosphorylation events in signaling pathways, cancer research, and immune regulation.
By offering single-method or combination strategies, MtoZ Biolabs ensures optimal enrichment efficiency and reproducibility tailored to each project.
Figure 1. Workflow of Phosphopeptide Enrichment
Why Choose MtoZ Biolabs?
✔ Multiple Enrichment Strategies: TiO₂, IMAC, SCX, and antibody-based methods tailored to diverse projects.
✔ High Sensitivity and Specificity: Optimized protocols maximize recovery of low-abundance phosphopeptides.
✔ Customizable Workflows: Flexible combinations of enrichment strategies ensure comprehensive results.
✔ Integration with Downstream Services: Seamless transition to phosphorylation site identification, quantitative analysis, and bioinformatics.
✔ Proven Expertise: Extensive experience in phosphoproteomics research with standardized, reproducible workflows.
✔ Transparent Pricing: One-time charge with no hidden costs or unexpected fees.
Sample Submission Suggestions
MtoZ Biolabs accepts a wide range of biological materials for phosphopeptide enrichment:
1. Cells: Cultured cell lines or primary cells in pellet form
2. Tissues: Fresh or frozen tissue samples from animal or plant sources
3. Biofluids: Serum, plasma, cerebrospinal fluid, urine, and other fluids
4. Exosomes: Isolated extracellular vesicles from culture media or patient samples
5. Plant Materials: Leaf, root, or seed extracts for phosphorylation studies
For detailed submission guidelines, please contact MtoZ Biolabs.
Applications
The Phosphopeptide Enrichment Service plays a vital role in multiple areas of life sciences research:
1. Signal Transduction Studies: Mapping dynamic phosphorylation events across signaling cascades.
2. Cancer Research: Profiling oncogenic tyrosine phosphorylation for biomarker discovery and therapeutic development.
3. Drug Target Identification: Detecting kinase substrates and phosphorylation-regulated networks.
4. Biomarker Development: Capturing disease-associated phosphorylation patterns for diagnostics and prognosis.
5. Plant and Agricultural Research: Understanding phosphorylation-driven stress responses and metabolic regulation in crops.
Phosphopeptide Enrichment Service at MtoZ Biolabs ensures sensitive, reliable, and comprehensive capture of phosphorylated peptides using advanced and complementary enrichment methods. Contact us today to strengthen your phosphoproteomics research with our Phosphopeptide Enrichment Service.