Lysine Leucylation Proteomics Profiling Service

MtoZ Biolabs delivers a comprehensive Lysine Leucylation Proteomics Profiling Service, offering large-scale, proteome-wide investigation of lysine leucylation. Our advanced workflows incorporate high-resolution mass spectrometry and tailored enrichment strategies to identify, map, and quantify leucine-modified lysine residues across complex biological samples.

What Is Lysine Leucylation?

Lysine leucylation is a type of post-translational modification (PTM) in which a leucyl (leucine residue) or leucine-derived moiety is covalently attached to the ε-amino group of a lysine side chain. Although less well characterized than more common modifications such as acetylation or methylation, emerging research suggests that novel acylations or residue attachments on lysine, triggered by reactive acyl-CoA derivatives or enzymatic transfers, play significant regulatory roles in cell biology.

Lysine Leucylation Proteomics Profiling refers to the global analysis of this modification across an entire proteome. Rather than focusing on one protein or a few sites, this approach provides a broad map of lysine leucylation patterns, site occupancy, and modification dynamics in cells, tissues, or biofluids. Since leucylation is a less explored PTM, large-scale profiling opens avenues for novel functional insights in proteomics, structural biology, and translational research.

Lysine Leucylation Proteomics Profiling Service at MtoZ Biolabs

By combining robust workflows, high-resolution mass spectrometry, and powerful bioinformatics interpretation, MtoZ Biolabs delivers a complete solution for understanding lysine leucylation at the proteome scale, helping researchers uncover new layers of protein regulation and signaling complexity.

Our Services Include:

💠Proteome-wide identification of leucylation sites across thousands of proteins in cells, tissues, or biofluids.

💠Quantitative profiling of modification dynamics under different biological or experimental conditions using label-free or labeling strategies such as TMT or SILAC.

💠Functional enrichment and pathway analysis to reveal biological processes and molecular networks regulated by lysine leucylation.

💠Comparative studies between disease and control samples to discover differential modification patterns or novel regulatory signatures.

Workflow of Lysine Leucylation Proteomics Profiling Service

1. Sample Preparation

• Receive and evaluate sample type, purity, protein concentration, and buffer compatibility
• Prepare proteins by reduction, alkylation, and enzymatic digestion

2. Peptide Enrichment

• Implement capture workflow to isolate leucylated peptides
• Wash and elute enriched peptides for MS analysis

3. LC-MS/MS Acquisition

• Use nanoLC to separate peptides
• Acquire MS and MS/MS spectra with high mass accuracy to detect leucyl-modified peptides and identify exact modification sites

4. Data Analysis

• Process raw data using advanced software
• Assign modification sites, quantify occupancy, and validate results
• Perform statistical comparison between sample groups

5. Report Generation

• Deliver tables of identified sites, modification occupancy, peptides, and quantitative differences
• Provide visualizations (chromatograms, spectra) and interpretation of biological significance

Why Choose MtoZ Biolabs

✅ Broad Proteome Coverage: Our workflows capture low-abundance leucylated peptides across complex samples, enabling wide proteomic coverage and discovery.

✅ High Modification Specificity: Enrichment methods coupled with high-resolution MS provide precise site localization and quantification of leucylation.

✅ Quantitative Flexibility: Supports both label-based and label-free quantification strategies, allowing tailored comparative analysis of modification changes.

✅ Customizable Experimental Design: We design bespoke workflows based on sample type, experimental question, and PTM profiling needs.

✅ Expert Analysis and Reporting: Our scientific team offers deep interpretation of results, linking leucylation sites to protein function and pathways.

Applications of Lysine Leucylation Proteomics Profiling Service

1. Metabolic Pathway Research

Explore how leucylation of lysine links branched-chain amino acid metabolism to protein regulation and signaling.

2. Disease Biomarker Discovery

Identify leucylation changes in disease models (e.g., cancer, metabolic disorders, aging) for potential diagnostic or therapeutic targets.

3. Proteome-Wide PTM Studies

Integrate leucylation profiling with other PTMs to reveal cross-talk and global modification networks.

4. Drug Development and Toxicology

Monitor how treatments or stressors affect lysine leucylation patterns, helping to assess mechanism of action or off-target effects.

5. Systems Biology

Map large-scale changes in leucylation across samples or conditions to inform functional network analyses and pathway mapping.

FAQ

Q1: What types of samples are suitable?

MtoZ Biolabs accepts a wide range of biological samples, including but not limited to:

• Blood or other biological fluids
• Animal and plant tissues
• Cell pellets or lysates
• Microbial cultures
• Purified proteins

For other sample types, please contact us in advance for tailored preparation guidance.

Q2: How should I prepare my samples?

1. Ensure samples are freshly prepared or snap-frozen to preserve protein modifications.

2. Avoid using detergents, reducing agents, or preservatives that may interfere with LC-MS/MS analysis.

3. Store samples at –80 °C before shipment.

4. Transport samples on dry ice to maintain stability during delivery.

For more information, please refer to Sample Submission Guidelines for Proteomics.

Q3: What is the service general workflow?

 

Q4: What data formats are provided?

• Raw LC-MS/MS data files compatible with major analysis platforms.
• Processed identification and quantification tables in CSV or Excel format.
• Annotated spectra and peptide–protein mapping summaries in PDF.
• Bioinformatics reports including pathway and enrichment results.
• Additional formats can be provided upon request to meet specific analysis or publication requirements.

Start Your Project with MtoZ Biolabs

Contact us to discuss your experimental design or request a quote. Our technical specialists are available to provide a free business assessment.