Modified Peptide Enrichment Service
Post-translational modifications (PTMs) are key mechanisms regulating protein function and cellular signaling networks. Many PTMs, including phosphorylation, acetylation, methylation, glycosylation, and ubiquitination, play essential roles in cell growth, differentiation, metabolism, and disease progression. However, these modifications are often low in abundance and highly dynamic, making it difficult to achieve comprehensive detection through MS alone.
Brandi, J. et al. J Chromatogr A. 2022.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. We offer a systematic modified peptide enrichment service based on advanced MS platforms and optimized sample preparation strategies. Through a combination of enrichment strategies, we help researchers analyze modified peptides at multiple levels, facilitating in-depth studies of signaling pathways, protein regulation, and disease mechanisms.
Services at MtoZ Biolabs
The core of the modified peptide enrichment service is to capture targeted modified peptides using selective strategies, thereby reducing background interference and improving specificity. At MtoZ Biolabs, we offer a wide range of enrichment approaches to meet different research needs:
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High-specificity antibodies are used to recognize particular modifications (e.g., phosphorylation, ubiquitination, acetylation), enabling targeted capture of modified peptides. This approach is suitable for site-specific or key modification studies. |
Immobilized Metal Affinity Chromatography (IMAC/TiO₂ Enrichment) Widely used for phosphopeptide enrichment, based on interactions between metal ions and negatively charged phosphate groups. It is well-suited for high-throughput and broad coverage. |
Lectin Affinity Chromatography Designed for glycopeptide enrichment, leveraging lectins that specifically bind to glycan moieties, thereby increasing the detection rate of glycosylated peptides. |
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Chemoselective Probes Capture peptides with specific reactive groups such as hydroxyl, carboxyl, or amine modifications. This is useful for studying niche or emerging types of PTMs. |
Metabolic and Chemoenzymatic Labeling Introduces detectable chemical tags through metabolic incorporation or enzymatic modification, enabling specific capture of modified peptides and high-resolution MS analysis. |
Pull-Down Enrichment Uses biotinylation or specific ligands to capture modification-associated protein complexes, suitable for exploring interactions related to PTMs. |
Fractionation and Preprocessing Strategies
To reduce sample complexity prior to enrichment, MtoZ Biolabs offers multiple fractionation approaches:
1. SCX (Strong Cation Exchange) and SAX (Strong Anion Exchange): Separate peptides by charge state, increasing modification coverage.
2. ERLIC (Electrostatic Repulsion Hydrophilic Interaction Chromatography): Suitable for acidic modified peptides.
3. HILIC (Hydrophilic Interaction Chromatography): Provides advantages in glycopeptide and polar PTM analysis.
4. Offline Basic RPLC Fractionation: Reduces complexity and improves depth of analysis when combined with enrichment.
The "fractionation + enrichment" strategy at MtoZ Biolabs significantly increases the number and coverage of identified modified peptides, especially in complex samples such as tissues and plasma/serum.
In addition to diverse enrichment and fractionation methods, MtoZ Biolabs also provides downstream Post-Translational Modifications Proteomics Analysis, including precise site identification, quantitative comparison of modification levels, and functional pathway interpretation. This enables researchers to extend from modification detection to biological insights, achieving a complete workflow from sample to data interpretation.
Service Advantages
1. Diverse Enrichment Strategies
We offer multiple approaches including immunoaffinity, IMAC/TiO₂, lectin affinity, chemoselective probes, and metabolic labeling, ensuring broad coverage of different PTM types. Researchers can select the most suitable strategy based on their goals to achieve accurate PTM characterization.
2. Fractionation Plus Enrichment Strategy
By combining fractionation methods such as SCX and HILIC with enrichment, we significantly improve the detection rate and coverage of modified peptides, particularly in complex samples and low-abundance PTMs.
3. High Sensitivity and Specificity
Enrichment reduces sample background, enabling detection of low-abundance PTMs. Coupled with high-resolution MS, this ensures precise identification and quantification of modification sites with reliable results.
4. Flexible Customization
Experimental schemes are customized based on sample origin (cells, tissues, biofluids), PTM type, and research focus, ensuring alignment with specific scientific questions.
5. One-Time Charge
Our pricing is transparent, with no hidden fees or additional costs.
Sample Submission Suggestions
1. Sample Types: Cells, tissues, plasma/serum, or other protein-containing samples.
2. Sample Amount: Recommended 100–500 μg of total protein.
3. Storage and Shipping: Store at –80°C; ship on dry ice to ensure stability.
4. Freeze-Thaw: Avoid repeated freeze-thaw cycles.
5. Additional Information: Provide sample source, treatment details, and research objectives to optimize study design.
Deliverables
1. Complete raw MS data (RAW or mzML formats)
2. Identified peptide lists with modification site annotations
3. Quantitative results (e.g., abundance changes, statistical analysis)
4. Enrichment efficiency assessment report
5. Standardized experimental report, including methodology, QC information, and result summary
PTM research is a critical approach to understanding biological processes and disease mechanisms, and Modified Peptide Enrichment is the key step for high-sensitivity and systematic detection. With extensive experience and diverse enrichment strategies, MtoZ Biolabs delivers reliable and comprehensive modified peptide analysis solutions.
Contact our professional team today to advance your research to the next level.